Author:
Ainara Otamendi 1, Oier Etxebeste 1, Eduardo A. Espeso 2
Author address:
1 Laboratory of Biology, Department of Applied Chemistry, Faculty of Chemistry, University of The Basque Country (UPV/EHU), San Sebastian, Spain;
2 Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain
Full conference title:
15th European Conference on Fungal Genetics 2020
Date: 20 June 2020
Abstract:
Fungi spread to new niches and colonize them by producing thousands of asexual spores. Aspergillus nidulans is a primary reference species for the study of the genetic/ molecular mechanisms governing fungal asexual development (conidiation). The transcription factor BrlA plays a central role in this process since: 1) its levels are controlled by signal transducers collectively known as UDAs; and 2) it governs the expression of CDP regulators, which control the morphological transitions leading to conidia production. In response to the emergence of fungal cells to the air, the main stimulus triggering conidiation, UDA mutants such as the flbB deletant fail to induce brlA expression. Nevertheless, the need for FlbB activity can be bypassed by culturing ∆flbB colonies in a medium containing high concentrations of H2 PO4 – . We used this phenotypic trait and an UV-mutagenesis procedure to isolate and characterize ∆flbB mutants unable to conidiate in these conditions. The characterization of FLIP166 led to the identification of the putative transcription factor SocA as a multicopy suppressor and PmtC(P282L) as the recessive mutant form responsible for the Fluffy (aconidial) In Phosphate phenotype. Taken together, results validate this novel strategy to identify genes and/or mutations related to the control of asexual development.
Link to conference website:
Link Conference abstract:
Conference abstracts, posters & presentations
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Title
Author
Year
Number
Poster
-
v
Christoph Knuf
, Intawat Nookaew
and Jens Nielsen2012
49)
n/a
-
v
Ewald Priegnitz,
Andreas Wargenau,
Ulrike Brandt,
Manfred Rohde,
Sylvia Dietrich,
Rainer Krull,
André
Fleißner2012
PR8.15
n/a
-
v
A. Verma*, G. Auzinger, M. Kantecki, D. Spurden, S. Cooper,
N. Heaton2012
NULL
n/a
-
v
Isabelle Benoit,
Helena Culleton,
Ad Wiebenga,
Pedro M. Coutinho,
Carlo P.J.M. Brouwer,
Vincent McKie,
Barry McCleary,
Bernard Henrissat,
Ronald P. de Vries2012
PR8.66
n/a
-
v
A.P. Silva*, I. Faria-Ramos, S. Costa-de-Oliveira, C. Pina-Vaz,
A.G. Rodrigues2012
NULL
n/a
-
v
D. A. Macdonald, A. E. Johns, M. Eberle, P. Bowyer, D. Denning, M. J. Bromley
2013
N/A
n/a
-
v
O.A. Cornely*, B. Gachot, H. Akan, M. Bassetti, O. Uzun, C. Kibbler,
O. Marchetti, J. Bille, P. de Burghgraeve, L. Pylkkanen, L. Ameye,
M. Paesmans, J. Donnelly on behalf of the Infectious Diseases
Group (IDG of the European Organization for Resea2012
NULL
n/a
-
v
M. Siopi*, A. Elefanti, L. Zerva, J. Meletiadis
2012
NULL
n/a