) Expression Response Of Aspergillus oryzae To Different Nitrogen Sources In Batch Cultivations

Ref ID: 18298

Author:

Christoph Knuf
, Intawat Nookaew
and Jens Nielsen

Author address:

Systems and Synthetic Biology Group, Department of Chemical and
Biological Engineering, Chalmers University of Technology

Full conference title:

Asperfest 9

Abstract:

The filamentous fungus Aspergillus oryzae is widely used as a microbial cell factory for large scale heterologous protein production.
A. oryzae is also known as a natural organic acid producer. However, the metabolism and regulation of organic acid production in A.
oryzae are poorly characterized. Furthermore, the media composition has a major impact on the performance of this organism and the
economic feasibility of an industrial fermentation process. We therefore evaluated the global expression response towards and the
utilization of different nitrogen sources by A. oryzae in batch fermentations. In this study, we aim for a deep investigation of the
cellular mechanisms of the utilization of different nitrogen sources. Firstly, we performed batch cultivations with two strains
(NRRL3488 and DSM1862) on defined and complex nitrogen sources using di-ammonium sulphate and peptone, respectively. In
addition, transcriptome analysis was performed on samples from these fermentations to analyze the gene expression under exponential
growth conditions (mid-exponential phase; 6h) and in nitrogen starvation (stationary phase; 30h) to further identify key-players in the
metabolism and regulation of gene expression. Cluster analysis helped to identify regulatory sequences among the co- expressed
genes. Furthermore the expression data pointed towards malic acid production as a response to nitrogen starvation stress.

Abstract Number: 49)

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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