Secondary metabolites, structure diagram: Trivial name – Dehydroaustin

Date: 26 November 2013

Secondary metabolites, structure diagram: Trivial name – Dehydroaustin

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Notes:

Species: A. niger, A. stellatus, A. ustus, Emericella dentataSystematic name: Spiro[1H,4H,8H-3a,7a-epoxy-6,11b-methano-3H-furo[3,4-e][3]benzoxocin-9(10H),3′(6’H)-[2H]pyran]-1,4,6′-trione, 7-(acetyloxy)-6,7,11,11a-tetrahydro-2′,2′,3,6,11a-pentamethyl-8,12-bis(methylene)-, (3R,3’R,3aR,6S,7R,7aR,11aR,11bS)-Molecular formulae: C27H30O9Molecular weight: 498Chemical abstracts number: 82893-35-4Selected references: Maebayashi, Yukio; Okuyama, Emi; Yamazaki, Mikio; Katsube, Yukiteru. Structure of ED-1 isolated from Emericella dentata. Chemical & Pharmaceutical Bulletin (1982), 30(5), 1911-12. Simpson, Thomas J.; Stenzel, Desmond J.; Bartlett, Alan J.; O’Brien, Eugene; Holker, John S. E. Studies on fungal metabolites. Part 3. Carbon-13 NMR spectral and structural studies on austin and new related meroterpenoids from Aspergillus ustus, Aspergillus variecolor, and Penicillium diversum. Journal of the Chemical Society, Perkin Transactions 1: Organic and Bio-Organic Chemistry (1972-1999) (1982), (11), 2687-92.


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  • A. versicolor by microscopy showing very long thin conidiophores.

    aspvers4

  • Pigmentation of Aspergillus versicolor colonies ranged from pale green to greenish-beige, pink-green, dark green and brown. Reverse is usually reddish. The growth rate is usually slow. Cultured on Sabouraud dextrose agar with chloramphenicol.

    aspvers3

  • A Colonies on MEA after one week; B, C conidial heads with tip of conidiophire, x920; D conidial head, x 2330; E conidial heads x920

    aspvers2

  • A Colonies on MEA + 20% sucrose after one week; B detail of colony showing columnar conidial heads x 44 ; C conidial heads x 920; D conidia x2330

    A

  • Scanning electron micrograph photo of conidia. Scale bar: 1 µm.

    piperis F

  • Conidia-forming head and culture plate

    A

  • Cultures are grown on malt extract agar for 5-7 days at 30°C.
    Light microscopy-1000x stained with lacto-phenol and cotton blue.

    niger

  • A Colonies on MEA +20% sucrose after one week; B ascomata x 40; C conidiophores x 920; D ascospores x2330; E ascoma x 230; F portion of ascoma with asci and ascospores, x 920.

    Aspergillus_chevalieri