Date: 26 November 2013
Pulmonary aspergillosis (cow 2). Section of lung from a 2 year old cow with weight loss and anorexia since calving. On necropsy examination, multiple firm masses were identified throughout the lungs. These were cavitating in nature, with a necrotic centre and peripheral fibrosis. Both this section and the following one are taken from the edge of such a lesion and demonstrate the pyogranulomatous inflammatory response.
Copyright:
© Dr. Michael Day, University of Bristol
Notes: n/a
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Pigmentation of Aspergillus versicolor colonies ranged from pale green to greenish-beige, pink-green, dark green and brown. Reverse is usually reddish. The growth rate is usually slow. Cultured on Sabouraud dextrose agar with chloramphenicol.
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A Colonies on MEA after one week; B, C conidial heads with tip of conidiophire, x920; D conidial head, x 2330; E conidial heads x920
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A Colonies on MEA + 20% sucrose after one week; B detail of colony showing columnar conidial heads x 44 ; C conidial heads x 920; D conidia x2330
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Cultures are grown on malt extract agar for 5-7 days at 30°C.
Light microscopy-1000x stained with lacto-phenol and cotton blue. -
A Colonies on MEA +20% sucrose after one week; B ascomata x 40; C conidiophores x 920; D ascospores x2330; E ascoma x 230; F portion of ascoma with asci and ascospores, x 920.