Date: 3 February 2014
D. 6 months later, tenacious yellow secretions in L basal bronchial division
Copyright:
(© Fungal Research Trust)
Notes:
Initially it was incorrectly diagnosed as a bronchial carcinoma.
The material was allergic mucin with mucus and cellular debris arranged in a layered pattern. Cellular debris was almost entirely eosinophils with scattered Charcot-Leyden crystals. A Grocott stain showed multiple branching fungal hyphae, consistent with Aspergillus spp. Subsequently her total IgE rose to 750 KIU/L and Aspergillus specific RAST to 14.9 KUa/L.
Images library
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Pigmentation of Aspergillus versicolor colonies ranged from pale green to greenish-beige, pink-green, dark green and brown. Reverse is usually reddish. The growth rate is usually slow. Cultured on Sabouraud dextrose agar with chloramphenicol.
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A Colonies on MEA after one week; B, C conidial heads with tip of conidiophire, x920; D conidial head, x 2330; E conidial heads x920
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A Colonies on MEA + 20% sucrose after one week; B detail of colony showing columnar conidial heads x 44 ; C conidial heads x 920; D conidia x2330
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Cultures are grown on malt extract agar for 5-7 days at 30°C.
Light microscopy-1000x stained with lacto-phenol and cotton blue. -
A Colonies on MEA +20% sucrose after one week; B ascomata x 40; C conidiophores x 920; D ascospores x2330; E ascoma x 230; F portion of ascoma with asci and ascospores, x 920.