Date: 26 November 2013
Four colonies of Aspergillus on an agar plate containing rose bengal (to limit colony spending) and elastin fibres (light pink dots). Underneath and surrounding the colonies, the elastin fibres have gone, indicating enzymatic degradation
Copyright:
© Denning DW, Elliott J, Keaney M. Temperature-dependent expression of elastase in Aspergillus species. J Med Vet Mycol, 1993;31:455-458.
Notes:
The medium was described by: Kothary MH, Chase T, Macmillan JD. Correlation of elastase production by some strains of Aspergillus fumigatus with ability to cause pulmonary invasive aspergillosis in mice. Infect Immun 1984; 43:320-3235.
Images library
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Legend
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Pigmentation of Aspergillus versicolor colonies ranged from pale green to greenish-beige, pink-green, dark green and brown. Reverse is usually reddish. The growth rate is usually slow. Cultured on Sabouraud dextrose agar with chloramphenicol.
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A Colonies on MEA after one week; B, C conidial heads with tip of conidiophire, x920; D conidial head, x 2330; E conidial heads x920
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A Colonies on MEA + 20% sucrose after one week; B detail of colony showing columnar conidial heads x 44 ; C conidial heads x 920; D conidia x2330
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Cultures are grown on malt extract agar for 5-7 days at 30°C.
Light microscopy-1000x stained with lacto-phenol and cotton blue.
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A Colonies on MEA +20% sucrose after one week; B ascomata x 40; C conidiophores x 920; D ascospores x2330; E ascoma x 230; F portion of ascoma with asci and ascospores, x 920.
