Itraconazole standards are placed in duplicate in a pre-arranged order and distributed throughout the plate. Patient samples of serum or plasma are placed in duplicate in other wells. A standard curve is drawn using the measured diameters of the standards and the patients’ samples then determined. The bioassay measures both itraconazole and the major bioactive metabolite of itraconazole – hydroxitraconazole.
Mucous containing Charcot-Leyden crystals, stained with H & EA 57 year old woman presented with breathlessness. She had a history of mild asthma for which she occasionally took salbutamol inhaler puffs. The patient underwent a pneumonectomy because of the severity of her disease process, and uncertainty about the diagnosis, prior to serology results being obtained.Serology showed an IgE of 2600, with a strongly positive Aspergillus RAST test and weakly positive Aspergillus precipitins. Material re
