Itraconazole standards are placed in duplicate in a pre-arranged order and distributed throughout the plate. Patient samples of serum or plasma are placed in duplicate in other wells. A standard curve is drawn using the measured diameters of the standards and the patients’ samples then determined. The bioassay measures both itraconazole and the major bioactive metabolite of itraconazole – hydroxitraconazole.
A four day A. fumigatus culture on malt extract agar (above). Light microscopy pictures are taken at 1000x mag., stained with lacto-phenol cotton blue (right).
A four day A. fumigatus culture on malt extract agar (above). Light microscopy pictures are taken at 1000x mag., stained with lacto-phenol cotton blue (right).
A four day A. fumigatus culture on malt extract agar (above). Light microscopy pictures are taken at 1000x mag., stained with lacto-phenol cotton blue (right).
![Asp[1]fumighead2](https://www.aspergillus.org.uk/wp-content/uploads/2013/11/Asp1fumighead2-150x150.jpg "Asp[1]fumighead2")
![Asp[1]fumighead](https://www.aspergillus.org.uk/wp-content/uploads/2013/11/Asp1fumighead-150x150.jpg "Asp[1]fumighead")
![Asp[1]fumigatus](https://www.aspergillus.org.uk/wp-content/uploads/2013/11/Asp1fumigatus-150x150.jpg "Asp[1]fumigatus")
