Date: 26 November 2013
Biofilms on bronchial epithelial cells in vitro.
Copyright:
Images kindly donated by Frank-Michael C. Müller, Pediatric Pulmonology, Cystic Fibrosis Centre and Infectious Diseases, Department of Pediatrics III, University of Heidelberg, Im Neuenheimer Feld 430, D-69120 Heidelberg, Germany.
Notes:
Confocal scanning laser microscopy (CLSM), using CAAF(green) Fun1(red) stained biofilm. The red color of the FUN 1 cell stain was localized in dense aggregates in the cytoplasm of metabolically active cells. Thus, areas of red fluorescence represented metabolically active cells, and green fluorescence indicated cell wall-like polysaccharides, while yellow areas represented dual staining.
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Image A
CT Scan 30/3/99
Showing extreme pleural thickening and 2 small cavities at apex of left lung.
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A 43 year old with smoking related emphysema was admitted to hospital with two separate episodes of haemoptysis. He had been in good health up to 1989, when he was diagnosed as having bilateral pulmonary tuberculosis. At that time a CT scan revealed a cavity in the left upper lobe (20.8cm2) with adjacent confluent infiltrates and pleural thickening. On bronchoscopic examination no abnormalities were noted and endobronchial biopsies did not reveal hyphae.
Over the next 4 years his condition deteriorated and a CT scan showed the left upper lobe cavity had increased to 40cm2. Itraconazole 400mg daily was prescribed. There was some clinical improvement on itraconazole but patient eventually deteriorated with breathlessness and with significant weight loss.
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