Date: 26 November 2013
Biofilms on bronchial epithelial cells in vitro.
Copyright:
Images kindly donated by Frank-Michael C. Müller, Pediatric Pulmonology, Cystic Fibrosis Centre and Infectious Diseases, Department of Pediatrics III, University of Heidelberg, Im Neuenheimer Feld 430, D-69120 Heidelberg, Germany.
Notes:
Confocal scanning laser microscopy (CLSM), using CAAF(green) Fun1(red) stained biofilm. The red color of the FUN 1 cell stain was localized in dense aggregates in the cytoplasm of metabolically active cells. Thus, areas of red fluorescence represented metabolically active cells, and green fluorescence indicated cell wall-like polysaccharides, while yellow areas represented dual staining.
Images library
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Title
Legend
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Necrotic lung tissue in culture
Af=Colony of Aspergillus fumigatus
B=bacterial colonies
L=lung tissue
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A photograph of part of the upper lung lobe of an immunosuppressed patient. The lung tissue shows extenive areas of necrosis due to invasive colonisation.
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A photograph of a cavity in the upper lobe of the lung of a patient with ankylosing spondylitis. Such cavitation,which may be confused with prior tuberculosis, can follow fibrosis.
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Plugs stained with Methenamine/silverActively growing mycelia of the fungus are a deep brown/black. Counterstaining shows the dense mucus of the plugs as predominantly orange.
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Sputum from an asthmatic patient showing plugs(casts). The development of plugs coincided with an increased prevalence and severity of episodes of asthma
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microscopic characters Conidiophore stipes(C)1300-2800um long:Vesicles(V)40-70um wide,clavate:Phialides(Ph) uniseriate:Conidia(Con)3.5-4.0um long,smooth walled.
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microscopic characters Conidiophore stipes(C)225-350um arising from hyphae(Hy):Vesicles(Ves)15-25um wide:Phialides(Ph)uniseriate:Conidia(Con)2.4-3.0um spherical to ovoid,roughened.
