Images kindly donated by Frank-Michael C. Müller, Pediatric Pulmonology, Cystic Fibrosis Centre and Infectious Diseases, Department of Pediatrics III, University of Heidelberg, Im Neuenheimer Feld 430, D-69120 Heidelberg, Germany.
Notes:
Confocal scanning laser microscopy – (CLSM). CAAF stained showing the hyphal network in contact with extracellular matrix. The polysaccharides of the ECM and fungal cell walls were stained by CAAF (concanavalin A-Alexa Fluor 488 conjugate).
Test for aflatoxin B1. Standard curve wells in triplicate – columns 9, 10 & 11 – laid out horizontally. Triplicates of 14 samples laid out vertically in rows 2 to 8
Light microscopical appearance of hepatic tissue in outbreak of aflatoxicosis showing centrilobular degeneration of hepatocytes with vacuolated cytoplasm and apoptoses (top right abnormal)(Haematoxylin and eosin).
Aspergillus terreus Thom. A Colonies on MEA after one week; B detail of colony showing columnar conidial heads x 44 ; C conidial heads and tip x 920; D conidia x2330
Aspergillus terreus Thom. Conidial head of Aspergillus terreus. Conidial heads are compact, columnar and biseriate. Conidiophores are hyaline to slightly yellow and smooth walled.