Description: A large family of techniques to achieve super-resolution imaging utilize single molecule switching and localization microscopy. In these techniques, such as STORM, PALM, FPALM, and GSDIM, super-resolution is achieved by first switching all the molecules in the sample to a non-fluorescent state. Individual molecules are then returned to the fluorescent state, imaged, and their position determined to much higher than the diffraction limit. This lecture describes these techniques, dye requirements (photoswitchable fluorescent proteins and small molecule dyes) and how to extend these techniques to 3 dimensional imaging.
About the Speaker: Bo Huang
Dr. Huang’s research focuses on using super-resolution microscopy and single-molecule imaging to understand how proteins form large complexes and how proteins interact to regulate signaling. Huang is an Assistant Professor in Pharmaceutical Chemistry and in Biochemistry and Biophysics at UC San Francisco.
For full tutorial and assessment go to iBiology
Medical and Patient education videos
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I have Sarcoidosis, Chronic Pulmonary Aspergillosis (CPA) and a very low CD4 count. I have challenged myself to do a daily vlog for 30 days
Follow me on twitter @StewArmstrong
Here is the link for all of the monthly aspergillosis meetings http://www.nacpatients.org.uk/monthly…
This meeting was for July 2016.
Aspergillus Website: Follow all of our Youtube collections here
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Stewart Armstrong has chronic pulmonary aspergillosis and has undertaken to record a video of every day of his life for a month in order to help raise awareness of aspergillosis and how it impacts their daily lives.
These videos are interesting for a number of reasons – not least because patients often say that they don’t look unwell so many people don’t understand how serious aspergillosis is. Does Stewart look or act unwell?
See the month of vlogs in the playlist below, or go to Stewart’s YouTube page to watch more of his videos.
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Global fungal killers and life-threatening infections
Fungi are everywhere, and a few species can cause very serious lethal infections. Fungal infections
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This panel considers and debates one of the greatest obstacles to food security in many parts of the world: mycotoxin. Aflatoxin is a particularly dangerous mycotoxin produced predominantly by two Aspergillus fungi. It colonizes a variety of important food and feed crops both pre- and post-harvest, including groundnuts, tree nuts, maize, rice, figs and other dried foods, spices, crude vegetable oils and cocoa. Contaminated crops have significant health risks for both humans and animals, having been linked to retarded growth and development (stunting), immunosuppression and liver cancer. The aflatoxin issue has other, complex implications for food security and, by limiting farmers’ access to international markets, can lead to food waste and economic instability.
Panelists:
-John Lamb, Principal Associate, Abt Associates
-Dr. Kitty Cardwell, National Program Leader, USDA-NIFA
-Barbara Stinson, Senior Partner, Meridian Institute; Project Director, Partnership for Aflatoxin Control in Africa
-Moderator: Dr. Howard-Yana Shapiro, Chief Agricultural Officer, Mars, Incorporated -
The role of poorly maintained ventilation ductwork in spreading airborne infections is largely ignored because it is ‘out of sight out of mind’, but recent analysis by healthcare professionals confirms that the risks are increasing.
Ghasson Shabha will identify particular threats in healthcare facilities from dirty ductwork. He also points out that fewer than 5% of building air-conditioning systems have been inspected despite regulations making this mandatory. Dr Shabha will look at how planned cleaning strategies can reduce risks and how hospitals can use 3D building information modelling software to identify ‘infection hotspots’.
The healthcare environment can be described as a reservoir for potentially infective agents which can spread unpredictably in a whole array of ways particularly in ventilation and air-conditioning systems making it difficult to effectively control and manage, for those seeking timely information about the patterns of cross-infection. The severity of the problem has been highlighted extensively over the past decade, as part of wider umbrella of what was known as health care acquired infections (HCAIs). Airborne transmission extends over a wide spectrum, and includes many prevalent agents, inter alia Mycobacterium tuberculosis (TB),nosocomial MRSA, Aspergillus fumigatus, Serratia marcescens, norovirus, and other airborne infection.
Dr Shabha was speaking live from Birmingham and this CIBSE ASHRAE Group (cibseashrae.org) webinar was co-sponsored by the B&ES Ventilation Hygiene Group and the Institute of Healthcare Engineering and Estate Management (IHEEM).
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Oliphant Science Awards – Year 1 project by Haruki Kurioka, Aleksa Novakovic and Bogdan Novakovic (St Andrew’s Primary School, Adelaide)
Food that we consume daily is rich in good bacteria and fungi. Yogurt, Yakult and Natto (fermented soybeans) are rich in bacteria. Cheeses (e.g. Brie & Blue cheese) and Koji contain fungi Penicillium and Aspergillus, respectively. Many Japanese and continental seasonings and dishes can be prepared with those products. Many kids and their parents don’t know much about good bacteria and fungi. First year students are here to tell us their story. Enjoy it!
