Description: A large family of techniques to achieve super-resolution imaging utilize single molecule switching and localization microscopy. In these techniques, such as STORM, PALM, FPALM, and GSDIM, super-resolution is achieved by first switching all the molecules in the sample to a non-fluorescent state. Individual molecules are then returned to the fluorescent state, imaged, and their position determined to much higher than the diffraction limit. This lecture describes these techniques, dye requirements (photoswitchable fluorescent proteins and small molecule dyes) and how to extend these techniques to 3 dimensional imaging.
About the Speaker: Bo Huang
Dr. Huang’s research focuses on using super-resolution microscopy and single-molecule imaging to understand how proteins form large complexes and how proteins interact to regulate signaling. Huang is an Assistant Professor in Pharmaceutical Chemistry and in Biochemistry and Biophysics at UC San Francisco.
For full tutorial and assessment go to iBiology
Medical and Patient education videos
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Title
Description
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Nasal Aspergillosis destroys the bone in and around the nose. Unfortunately this case could not be treated and the dog had to be euthanised.
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Nasal aspergillosis with moderate turbinate destruction and fungal plate on left frontal sinus mucosa. Biopsy was performed and 10% clotrimazole cream was applied through endoscope.
[Aspergilosis nasal con moderada destrucción de cornete etmoidal izquierdo y placa micótica sobre mucosa de seno frontal. Se realizó la biopsia y se aplicó una crema de clotrimazol al 10% mediante el endoscopio.]
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This video depicts removal of superficial fungal infection involving the right ear canal by using suctioning technique
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More examination & analysis of lung specimens from a patient with aspergillosis
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A histological tour of the signs of aspergillosis in lung specimens
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A collection of short video’s hosted by the Aspergillus Website showing various aspects of the cell biology of Aspergillus and how parts of our immune system fights the infection.
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Sir David Attenborough and the Planet Earth team discover the weird world of the Cordyceps; killer fungi that invades the body of an insect to grow and diminish the insect population. Fascinating animal and wildlife video from the BBC epic natural world masterpiece ‘Planet Earth’.
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This video was kindly provided with permission from Lariontsev Nikolay.
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A patient describes her experience of living with chronic pulmonary aspergillosis and treatment with itraconazole.
