Description: A large family of techniques to achieve super-resolution imaging utilize single molecule switching and localization microscopy. In these techniques, such as STORM, PALM, FPALM, and GSDIM, super-resolution is achieved by first switching all the molecules in the sample to a non-fluorescent state. Individual molecules are then returned to the fluorescent state, imaged, and their position determined to much higher than the diffraction limit. This lecture describes these techniques, dye requirements (photoswitchable fluorescent proteins and small molecule dyes) and how to extend these techniques to 3 dimensional imaging.
About the Speaker: Bo Huang
Dr. Huang’s research focuses on using super-resolution microscopy and single-molecule imaging to understand how proteins form large complexes and how proteins interact to regulate signaling. Huang is an Assistant Professor in Pharmaceutical Chemistry and in Biochemistry and Biophysics at UC San Francisco.
For full tutorial and assessment go to iBiology
Medical and Patient education videos
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An interesting description of how a skin prick test is carried out with the intention of finding out which substances a patient is allergic to. Amongst other substances Aspergillus is tested for in this video.
This type of test is quick and easy to carry out and can be useful when screening a large number of allergens. More detailed description
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The CF Discovery SeriesTM is an informative and engaging program designed to educate and uplift those with cystic fibrosis. Through interactive presentations with experts in the field, those living with cystic fibrosis gain information in a setting which encourages camaraderie and community. You can attend these events in person. Guests attending in person must follow CFRI’s Infection Control Policy, found here.
