Description: A large family of techniques to achieve super-resolution imaging utilize single molecule switching and localization microscopy. In these techniques, such as STORM, PALM, FPALM, and GSDIM, super-resolution is achieved by first switching all the molecules in the sample to a non-fluorescent state. Individual molecules are then returned to the fluorescent state, imaged, and their position determined to much higher than the diffraction limit. This lecture describes these techniques, dye requirements (photoswitchable fluorescent proteins and small molecule dyes) and how to extend these techniques to 3 dimensional imaging.
About the Speaker: Bo Huang
Dr. Huang’s research focuses on using super-resolution microscopy and single-molecule imaging to understand how proteins form large complexes and how proteins interact to regulate signaling. Huang is an Assistant Professor in Pharmaceutical Chemistry and in Biochemistry and Biophysics at UC San Francisco.
For full tutorial and assessment go to iBiology
Medical and Patient education videos
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Title
Description
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European Respiratory Society: Current thinking regarding the importance of nutritional science in the management and prevention of COPD.
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European Respiratory Society: Should we be testing new drugs in the ‘real’ world where some patients smoke, are obese and have other comorbidities?
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European Respiratory Society: A revolutionary collaborative approach to research funding is providing new insights into the origins of chronic respiratory diseases.
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European Respiratory Society: Clean air is vitally important for people who live with aspergillosis. Chemical pollutants have the capacity to worsen symptoms and quality of life, PM2.5 particulates (mentioned in this video) include aspergillus and other fungal spores.
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European Respiratory Society launches its latest vision to support those with respiratory health difficulties throughout europe: encouraging patients to increase their levels of activity which is proven to improve lung health.
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Stewart Armstrong inspiring many people to do ‘Push-ups for aspergillosis’ to raise awareness and funds for aspergillosis research.
Facebook page (November 2015)
Also see the compelling video of Stewarts’ story as told by himself
