Description: A large family of techniques to achieve super-resolution imaging utilize single molecule switching and localization microscopy. In these techniques, such as STORM, PALM, FPALM, and GSDIM, super-resolution is achieved by first switching all the molecules in the sample to a non-fluorescent state. Individual molecules are then returned to the fluorescent state, imaged, and their position determined to much higher than the diffraction limit. This lecture describes these techniques, dye requirements (photoswitchable fluorescent proteins and small molecule dyes) and how to extend these techniques to 3 dimensional imaging.
About the Speaker: Bo Huang
Dr. Huang’s research focuses on using super-resolution microscopy and single-molecule imaging to understand how proteins form large complexes and how proteins interact to regulate signaling. Huang is an Assistant Professor in Pharmaceutical Chemistry and in Biochemistry and Biophysics at UC San Francisco.
For full tutorial and assessment go to iBiology
Medical and Patient education videos
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Title
Description
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Talk by Gilbert Massard – at the Aspergillosis for Patients meeting in Rome, Feb 3rd 2010
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Talk by David Andes – at the Aspergillosis for Patients meeting in Rome, Feb 3rd 2010.
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Talk by Brahm Segal – at the Aspergillosis for Patients meeting in Rome, Feb 3rd, 2010.
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Talk by David Denning – at the Aspergillosis for Patients meeting in Rome, Feb 3rd, 2010.
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Talk by Russell Lewis – at the Aspergillosis for Patients meeting in Rome, Feb 3rd, 2010.
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An introduction to this meeting by Dr Geoffrey Scott, Consultant Microbiologist.
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Talk by Rick Moss – at the Aspergillosis for Patients meeting in Rome, Feb 3rd, 2010.
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Talk by Malcolm Richardson – at the Aspergillosis for Patients meeting in Rome, Feb 3rd, 2010, with specific references to aspergillus.
