Description: A large family of techniques to achieve super-resolution imaging utilize single molecule switching and localization microscopy. In these techniques, such as STORM, PALM, FPALM, and GSDIM, super-resolution is achieved by first switching all the molecules in the sample to a non-fluorescent state. Individual molecules are then returned to the fluorescent state, imaged, and their position determined to much higher than the diffraction limit. This lecture describes these techniques, dye requirements (photoswitchable fluorescent proteins and small molecule dyes) and how to extend these techniques to 3 dimensional imaging.
About the Speaker: Bo Huang
Dr. Huang’s research focuses on using super-resolution microscopy and single-molecule imaging to understand how proteins form large complexes and how proteins interact to regulate signaling. Huang is an Assistant Professor in Pharmaceutical Chemistry and in Biochemistry and Biophysics at UC San Francisco.
For full tutorial and assessment go to iBiology
Medical and Patient education videos
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News article made for the regional BBC TV program North West Tonight during the 7th Advances Against Aspergillosis Meeting in Manchester, UK. March 3rd 2016.
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Dr Ritesh Argawal discusses fungal sinusitis and allergic bronchopulmonary aspergillosis (ABPA) with patients.
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Dr Richard Moss discusses allergic Aspergillosis, Asthma, children affected by Aspergillus + Q & A with patients.
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Darius Armstrong-James discusses a new aspergillosis clinic in the South East + Q & A with patients.
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BBC ran an awareness story during the Advances Against Aspergillosis conference in Manchester, UK about the dangers to our health posed by Aspergillus and some of the most common ways the fungus can grow and multiply in our homes. Ann Saunders and Prof David Denning, Director of the National Aspergillosis Centre helped discuss the issues.
Estimated audience at 7.40am is 1.5 million.
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The international Advances Against Aspergillosis conference came to Manchester this year (2016) and brought together the aspergillosis medical, scientific and patient communities for the first time in two years. Much progress was discussed and reported, awareness reaching 5 million people via UK TV BBC1 during the meeting.
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ERS Vision: Take the Active Option: the benefits of exercise in respiratory medicine
