Neutrophils cross epithelial borders and migrate on the alveolar surface an infected lung slice.

A Lys-EGFP mouse was treated as described for movie 4. The sequence shows several neutrophils (green) migrating within the lung slice. At 12 minutes into the movie a single cell is seen (at 5 o’ clock) crossing the epithelial border of an alveolus (blue) and migrating on its surface. Red shows DNA of nuclei (Sytox Orange). Indicated is a scale bar and real time in minutes.
Reference: Production of Extracellular Traps against Aspergillus fumigatus In Vitro and in Infected Lung Tissue Is Dependent on Invading Neutrophils and Influenced by Hydrophobin RodA. Bruns et. al. PLoS Pathog. 2010 Apr 29;6(4):e1000873.

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