Isolated neutrophils were incubated together with hyphae in the presence of the blue DNA dye Hoechst in a laboratory made incubation chamber and analysed by live cell microscopy. The movie shows how a number of free neutrophils associate with a small hyphal fragment and transport it to an area, where a larger hypha is already associated with many other neutrophils. Similar phenomena can be observed in acute lung slices. Real time is given at the bottom of the movie.
Reference: Production of Extracellular Traps against Aspergillus fumigatus In Vitro and in Infected Lung Tissue Is Dependent on Invading Neutrophils and Influenced by Hydrophobin RodA. Bruns et. al. PLoS Pathog. 2010 Apr 29;6(4):e1000873.
http://www.plospathogens.org/article/…
Medical and Patient education videos
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Title
Description
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Dr. Rohini Manuel, Consultant Clinical Microbiologist, Public Health England, London
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Shila Seaton, Bacteriology Scheme Manager, UK NEQAS for Microbiology, Public Health England
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Dr. P. Lewis White, Principal Clinical Scientist, Public Health Wales Microbiology, Cardiff
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Prof. Dr. Clemens Decristoforo, Radiopharmacist, Univ.Klinik f.Nuklearmedizin, Innsbruck, Austria
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Dr. Martin Hoenigl, Infectious Diseases and Tropical Medicine, Medical University of Graz, Austria
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Dr. Jonathan Lambourne, Consultant in Infectious Diseases, Barts Health NHS Trust, London
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Dr. Inês Ushiro-Lumb, Lead Clinical Microbiologist for Organ Donation and Transplantation, NHS Blood and Transplant, London
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Dr. Mike Bromley, Lecturer, Institute of Inflammation and Repair, University of Manchester
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Dr. Sharleen Braham, Clinical Scientist, King’s College Hospital, London
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Dr. Duncan Wilson, Research Fellow, Aberdeen Fungal Group, University of Aberdeen
