Description: Selective Plane Illumination Microscopy (SPIM) greatly reduces phototoxicity in comparison with other fluorescent imaging modalities and makes it possible to image living small animals in 3D over extended periods of time. This talk describes an extension of SPIM such that it can be used with specimens on a coverslip rather than in a capillary (inverted SPIM or iSPIM), and a second modification that images the specimen using two perpendicular light sheets (Dual-View iSPIM or diSPIM), resulting in 3D datasets with the same resolution in X, Y, and Z (isotropic resolution)
About the Speaker: Hari Shroff
Hari Shroff is an Investigator at the National Institute of Biomedical Imaging and Bioengineering at the National Institutes of Health. During his post-doc, which was with Eric Betzig at HHMI’s Janelia Farm Research Campus, he focused on the development of PALM (photoactivated localization microscopy) microscopy. Since then he has developed several microscopy techniques, such as diSPIM and instant structured illumination microscopy.
For full tutorial & assessment go to iBiology
Medical and Patient education videos
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Title
Description
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Nasal Aspergillosis destroys the bone in and around the nose. Unfortunately this case could not be treated and the dog had to be euthanised.
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Nasal aspergillosis with moderate turbinate destruction and fungal plate on left frontal sinus mucosa. Biopsy was performed and 10% clotrimazole cream was applied through endoscope.
[Aspergilosis nasal con moderada destrucción de cornete etmoidal izquierdo y placa micótica sobre mucosa de seno frontal. Se realizó la biopsia y se aplicó una crema de clotrimazol al 10% mediante el endoscopio.]
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This video depicts removal of superficial fungal infection involving the right ear canal by using suctioning technique
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More examination & analysis of lung specimens from a patient with aspergillosis
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A histological tour of the signs of aspergillosis in lung specimens
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A collection of short video’s hosted by the Aspergillus Website showing various aspects of the cell biology of Aspergillus and how parts of our immune system fights the infection.
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Sir David Attenborough and the Planet Earth team discover the weird world of the Cordyceps; killer fungi that invades the body of an insect to grow and diminish the insect population. Fascinating animal and wildlife video from the BBC epic natural world masterpiece ‘Planet Earth’.
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This video was kindly provided with permission from Lariontsev Nikolay.
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A patient describes her experience of living with chronic pulmonary aspergillosis and treatment with itraconazole.
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