Targeting Conserved Genes in Aspergillus Species

Detail:

Series: Methods in Molecular Biology | Volume: 1542 | Pub. Date: Dec-11-2016 | Page Range: 131-140 | DOI: 10.1007/978-1-4939-6707-0_7

Year prepared: 2016

The genus Aspergillus is among the economically most important fungal genera, which contains about 350 species. They occur worldwide, and have both beneficial and harmful effects on humans, animals, and plants. Several molecular sequence-based approaches have been tested to identify Aspergillus isolates at the species level. In this chapter, we give an overview of the methods which proved to be most suitable in our experience.

Laboratory Protocols

Select from:Additional methods & referenceAflatoxinsAssaying antifungal levelsAssaying mycotoxinsAztec Labs Diagnostic testing ProtocolsBiochemical CalculatorsBiofilmBiotypingCell Component ExtractionClinical BreakpointsCollection of Clinical MaterialCosmidsDetection in clinical samplesDNA extractionEnzyme synthesisFungal Molecular BiologyGeneral Molecular BiologyGeneticsGrowth and StorageHistologyMethods for DNA sequencingMicroscopyModel systems: in vitroModel systems: in vivoMore protocol collectionsPCR  -RT-qPCRPhagocytosisPlanar liquid chromatographyPost Antifungal Effects (PAFE)Preparation of amphotericin pasteProtein methodsProtocol overviewsQuality control in mycologySampling Aspergillus sporesSecondary metabolitesSpecies/Strain identification  -In situ hydridizationStandard Operating Procedures:National Aspergillosis CentreSusceptibility testingVeterinaryVideo Protocols at the FGSCWhole blood fractionationwhole-cell screening