Protein Extraction from Fungi

Detail:

Series: Methods in Molecular Biology | Volume: 59 | Pub. Date: Mar-22-1996 | Page Range: 39-48 | DOI: 10.1385/0-89603-336-8:39

Year prepared: 1996

In order to study proteins from yeasts and filamentous fungi, it is important to consider a number of basic features of the organisms. First, filamentous fungi undergo a growth cycle that includes differentiation and compartmentalization. In addition, both the filamentous fungi and yeasts will age during growth, and older cultures will undergo autolysis. As a result, particular proteins may only be associated with one part of the growth cycle, such as sporulation or autolysis, and this must be taken into account in determining growth conditions and sampling times.

Laboratory Protocols

Select from:Additional methods & referenceAflatoxinsAssaying antifungal levelsAssaying mycotoxinsAztec Labs Diagnostic testing ProtocolsBiochemical CalculatorsBiofilmBiotypingCell Component ExtractionClinical BreakpointsCollection of Clinical MaterialCosmidsDetection in clinical samplesDNA extractionEnzyme synthesisFungal Molecular BiologyGeneral Molecular BiologyGeneticsGrowth and StorageHistologyMethods for DNA sequencingMicroscopyModel systems: in vitroModel systems: in vivoMore protocol collectionsPCR  -RT-qPCRPhagocytosisPlanar liquid chromatographyPost Antifungal Effects (PAFE)Preparation of amphotericin pasteProtein methodsProtocol overviewsQuality control in mycologySampling Aspergillus sporesSecondary metabolitesSpecies/Strain identification  -In situ hydridizationStandard Operating Procedures:National Aspergillosis CentreSusceptibility testingVeterinaryVideo Protocols at the FGSCWhole blood fractionationwhole-cell screening