Methods for DNA isolation (E.coli) – Biomek protocol for 96 DNA preps

ID: 76

Group:

General Molecular Biology

Prepared by:

Bruce A. Roe (broe@uoknor.edu)

Detail:

This is an automated version of the double-stranded template DNA isolation procedure that has evolved in my laboratory over the past several years. This Biomek 2000 version was developed, programmed, and tested by Judy Crabtree. It has been used routinely for template isolations for over 6 months and thanks go to Adonis Reece, Guozhang Zhang and Steve Toth for their suggested modifications during the testing phase. With this protocol, a single Biomek 2000 can isolate four sets of 96 double-stranded DNA sequencing templates in less than 4 hours.

Year prepared: NULL

Date uploaded: 2010-02-26 14:20:42

Laboratory Protocols

Select from:Additional methods & referenceAflatoxinsAssaying antifungal levelsAssaying mycotoxinsAztec Labs Diagnostic testing ProtocolsBiochemical CalculatorsBiofilmBiotypingCell Component ExtractionClinical BreakpointsCollection of Clinical MaterialCosmidsDetection in clinical samplesDNA extractionEnzyme synthesisFungal Molecular BiologyGeneral Molecular BiologyGeneticsGrowth and StorageHistologyMethods for DNA sequencingMicroscopyModel systems: in vitroModel systems: in vivoMore protocol collectionsPCR  -RT-qPCRPhagocytosisPlanar liquid chromatographyPost Antifungal Effects (PAFE)Preparation of amphotericin pasteProtein methodsProtocol overviewsQuality control in mycologySampling Aspergillus sporesSecondary metabolitesSpecies/Strain identification  -In situ hydridizationStandard Operating Procedures:National Aspergillosis CentreSusceptibility testingVeterinaryVideo Protocols at the FGSCWhole blood fractionationwhole-cell screening