A rapid method for isolation of total nucleic acids from Aspergillus nidulans.

Detail:

With the development of molecular biology techniques and their application to the analysis of cellular events, the isolation of total nucleic acids in Aspergillus nidulans for Southern and Northern hybridization has become routine. The current methods of nucleic acids isolation in A. nidulans, however, are relatively tedious and limited to a small number of samples each time. For example, the method for nucleic acid isolation of Oakley et al. (1987 Gene 61:385-399) requires the grinding of mycelia, either in frozen or lyophilized form, before extraction with phenol. This method requires a relatively large amount of mycelium and up to a day of preparing the mycelium before the actual extraction of nucleic acids. To increase the efficiency so that many samples can be studied at the same time, we have modified a method for nucleic acids isolation from Saccharomyces cerevisiae (Elder et al. 1983 Proc. Natl. Acad. Sci. (USA) 80:1194-1198) and adapted it to A. nidulans.

Laboratory Protocols

Select from:Additional methods & referenceAflatoxinsAssaying antifungal levelsAssaying mycotoxinsAztec Labs Diagnostic testing ProtocolsBiochemical CalculatorsBiofilmBiotypingCell Component ExtractionClinical BreakpointsCollection of Clinical MaterialCosmidsDetection in clinical samplesDNA extractionEnzyme synthesisFungal Molecular BiologyGeneral Molecular BiologyGeneticsGrowth and StorageHistologyMethods for DNA sequencingMicroscopyModel systems: in vitroModel systems: in vivoMore protocol collectionsPCR  -RT-qPCRPhagocytosisPlanar liquid chromatographyPost Antifungal Effects (PAFE)Preparation of amphotericin pasteProtein methodsProtocol overviewsQuality control in mycologySampling Aspergillus sporesSecondary metabolitesSpecies/Strain identification  -In situ hydridizationStandard Operating Procedures:National Aspergillosis CentreSusceptibility testingVeterinaryVideo Protocols at the FGSCWhole blood fractionationwhole-cell screening