Transcription factor HSF1 decreases the expression of Surfactant Protein-D in cells infected with Aspergillus fumigatus.

Author:

SS Kim1, HJ Kim1, GS Shin2

Author address:

1Department of Biomedical Laboratory Science, Daejeon University, Daejeon, Korea
2Department of Life Science, Daejeon University, Daejeon, Korea

Full conference title:

9th Advances Against Aspergillosis

Date: 26 February 2020

Abstract:

Purpose: Aspergillosis is a life-threatening disease in patients with compromised immune systems. Fungal invasion processes are a highly critical step during host cellular infection. Previously, we suggested that Surfactant Protein-D (SP-D) is a protein suppressed by A. fumigatus conidia directly. We investigate what transcription factor and where the promoter region of SP-D is involved in the infection process of conidia-treated cells.

Methods: To investigate the promoter activity of SP-D in fungal infected cells, we cloned the different lengths of the promoter region (-1000 to +1) of SP-D and examined promoter activity in A. fumigatus conidia-treated A549 cells. Then, we assayed the activity of the promoter region of Surfactant Protein-D shows a response to conidia treatment. And then, we predicted what transcription factor is binding and what binding sites in the promoter regions of SP-D was involved using AliBaba 2.1 software. Using decoy assay, we confirmed the transcription factor is bind to the selected region directly.

Results: Previously, we showed that the promoter activity of SP-D is down-regulated to the conidia treatment. Promoter region from -200 to -100 bp of SP-D is the important region to response in the treatment of conidia. We refine the map to elucidate the core region (-150/-126) involved in mediating the conidia-induced SP-D promoter activity. Using AliBaba2.1 software, we select some candidate transcription factors in the promoter region of SP-D that responds to infection of conidia. And, we know the transcription factor HSF1 is located in the response region (-142 to -134 bp). The decoy assay result shows that transcription factor HSF1 is sufficient to decrease the expression of the SP-D gene.

Conclusion: We show that HSF1 as a negative regulator binds the promoter region of SP-D in conidia treated cells. We confirm that HSF1 suppresses the expression of SP-D in conidia-infected cells using decoy assay. We suggest that inhibition of HSF1 binding to the promoter region of SP-D is an important step to inhibit the infection of conidia.

Abstract Number: 34

Link to conference website:

Link Conference abstract: 

AAAM 2020

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