Release of Th2 tissue cytokines and BAFF by epithelial cells of nasal polyps

Author:

Boita, M 1; Bucca, C 1; Heffler, E 1; Riva, G 2; Rolla, G 1

Author address:

1 Medical Science, University of Turin, Turin, Italy; 2 ENT Division, University of Turin, Turin, Italy

Full conference title:

European Academy of Allergy and Clinical Immunology Congress 2015

Date: 5 August 2020

Abstract:

Background: The epithelial barrier is thought to play an important role in the onset of TH2-inflammatory response of chronic rhinosinusitis with nasal polyps (CRSwNP) through the release of tissue cytokines (TSLP, IL25, IL33). Some experimental data suggest that epithelial cells may participate in the local B cell proliferation and activation, with IgE production, through the release of B cell activating factors (BAFF). The aim of the study was to investigate the release of TSLP, IL25, IL33 and BAFF by epithelial cells derived by nasal polyps of patients with CRSwNP.

Method: Epithelial cells of NP (9 patients, 6 atopic) and inferior turbinate (IT) of CRSsNP (7 patients, 2 atopic) were cultured serum free under 24 h stimulus of Staphylococcal enterotoxin beta (SEB), D. pteronyssinus, Aspergillus fumigatus, and Poly I:C. IL-25, IL-33, TSLP and BAFF have been measured in supernatant by ELISA, in unstimulated condition and after the above reported stimuli.

Results: IL25 release was significantly increased by A. fumigatus in CRSsNP (from 0.18  0.07 pg/mL to 0.51  0.1 pg/ mL, P < 0.001) and by Poly I:C stimulation in patients with CRSwNP (from 0.12  0.06 pg/mL to 0.27  0.1 pg/mL, P < 0.01), with higher levels in atopic compared to non atopic patients (0.37  0.1 pg/mL vs 0.19  0.1 pg/mL, P < 0.05). IL33 release was significantly increased by D. pteronyssinus stimulation in CRSsNP (from 2.57  1.3 pg/mL to 5.7  3.1 pg/ mL, P < 0.001) with no differences between atopic and not atopic subjects. TSLP release was significantly increased after Poly I:C stimulation in CRSwNP (from 0.77  0.5 pg/mL to 2.53  1.17 pg/ mL, P < 0.001), with no differences between atopic and not atopic subjects. BAFF release was significantly increased at baseline in CRSwNP compared to CRSsNP (from 9.63  4.2 pg/mL to 5.2  1 pg/mL, P < 0.05) with no change after any stimulation.

Conclusion: Epithelial cells from nasal polyps may induce Th2 immune response when stimulated directly by Poly I:C, which is structurally similar to RNAs derived from cells with viral infection or sterile tissue damage. On the other hand, epithelial cells from IT of CRsNP seem to be more sensitive to proteases, derived from Aspergillus species or D. pteronyssinus, which have been shown to induce IL33 release when administered directly to the tissue. The higher amount of BAFF released by epithelial cells of nasal polyps suggests that these cells may contribute to local B cells proliferation and activation, commonly observed in sinus biopsies of CRSwNP.

Abstract Number: 1071

Link to conference website:

Link Conference abstract: 

EAACI 2015

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