Nutrient transporter translocation to the plasma membrane via Golgi bypass in Aspergillus nidulans

Author:

Sofia Dimou, Olga Martzoukou, Mariangela Dionysopoulou, Vangelis Bouris,
Sotiris Amillis, George Diallinas

Author address:

National and Kapodistrian University of Athens, Biology, Athens, Greece

Full conference title:

15th European Conference on Fungal Genetics 2020

Date: 20 June 2020

Abstract:

Eukaryotic nutrient transporters, being polytopic transmembrane proteins, are thought to traffic from their site of synthesis, the ER, to the plasma membrane, through the Golgi, using the conventional vesicular trafficking pathway. Notably, however, current knowledge on the mechanism of membrane cargo secretion has been obtained by using mostly proteins that are polarly localized, and to our knowledge no report has ever shown formally the involvement of Golgi specifically in the PM localization of de novo made transporters. Here we show that in the Aspergillus nidulans several nutrient transporters follow an unconventional trafficking route that initiates at ER-exit sites (ERes) and requires clathrin and actin polymerization, but surprisingly, does not involve passage through the Golgi or other key effectors of conventional secretion (Rab11, AP1, microtubules or endosomes). Our findings will be discussed relative to other studies concerning unconventional trafficking routes in other systems and within a rationale on why transporter traffic bypasses the Golgi. Last but not least, we will propose that the trafficking mechanism uncovered here in a lower eukaryote might hold true for the sorting of nutrient transporters and other house-keeping non-polar cargoes in higher organisms.

Link to conference website:

Link Conference abstract: 

ECFG 15

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