In vitro activity of active ingredients of disinfectants against drug-resistant fungi

Author:

R. Stauf1
, D. Todt2
, E. Steinmann2
, P.-M. Rath3
, H. Gabriel4
, J. Steinmann5
, F.H.H. Brill4

Author address:

1
Institute For Clinical Hygiene, Medical Microbiology And Infectiology, Paracelsus Medical
University Nuremberg, Nuremberg, Germany, 2Department For Molecular And Medical Virology,
Ruhr-University Bochum, Bochum, Germany, 3
Institute Of Medical Microbiology, University
Hospital Essen, Essen, Germany, 4
Institute For Hygiene And Microbiology, Dr. Brill + Partner GmbH,
Hamburg, Germany, 5
Institute Of Clinical Hygiene, Medical Microbiology And Clinical Infectiology,
Klinikum Nuernberg, Nuremberg, Germany

Full conference title:

9th Trends in Medical Mycology Conference 2019

Date: 8 November 2019

Abstract:

Objectives: Prevention of immunosuppressed patients against fungal pathogens is essential in times of emergence of antifungal-resistant yeasts and moulds. Therefore, the use of fungicidal disinfectants is an important measure preventing infections. In this study, the fungicidal activity of common microbial agents of chemical disinfectants against antifungal-resistant and antifungal-susceptible yeasts and moulds was tested in comparison to the reference strains Candida albicans, Candida tropicalis, Aspergillus brasiliensis and Aspergillus niger. Methods: In total, nine clinical isolates from the fungal collection of the University Hospital Essen and four reference strains were included. Species identification was performed by morphological methods and sequencing of the internal transcribed spacer region (ITS). The antifungal susceptibility testing was performed via microdilution assay according to EUCAST standard 9.3 for moulds and 7.3.1 for yeasts. The sensitivity of clinical yeasts and moulds isolates in comparison to the reference isolates was tested in a quantitative suspension test against peracetic acid and ethanol as the common active ingredients of chemical disinfectants. Results: Ethanol was sufficiently active with a log10 reduction factor of ≥ 4.0 against all yeasts in a concentration of 50 % v/v within 1 min and against all moulds as 80 % solution within 1 min. Peracetic acid was active as 0.25 % solution in 5 min against all yeasts and as 0.5 % solution in 5 min against all moulds. Compared to the reference strains the clinical isolates showed similar or higher sensitivity to the active ingredients. In addition, antifungal-resistant strains exhibited equivalent sensitivity in vitro against ethanol and peracetic acid compared to the susceptible ones. Conclusion: Clinical fungal isolates including multidrug resistant ones did show a similar or higher sensitivity to active ingredients of disinfectants than the reference strains.

Abstract Number: P015

Conference Year: 2019

Link to conference website:

Link Conference abstract: 

TIMM 2019

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