Efficacy of micafungin solved in ethanol in the treatment of experimental Candida parapsilosis catheter infection using the antifungal-lock technique

Ref ID: 17777

Author:

J. Gavalda`*, C.M. Orbegozo, X. Gomis, E. Roselló, N. Larrosa,
B. Almirante, A. Pahissa

Author address:

Barcelona, ES)

Full conference title:

22nd European Congress of Clinical Microbiology and Infectious Diseases

Abstract:

Background: Candida species have the ability to form biofilms, what
impairs the treatment of infected catheters. When infection involves a
highly needed catheter might not be an option. Our aim was to evaluate
the efficacy of liposomal amphotericin B (L-AmB) or micafungin
(MICA) in the treatment of C. parapsilosis (CP) catheter infection (CI)
using the antifungal lock technique (ALT).
Methods: New Zealand rabbits were surgically implanted with a
silicone catheter through the jugular vein. After insertion the catheter
was locked with Antibiotic Medium nordm;3 (Oxoid) supplemented
with 7.5% dextrosa and 100 IU of heparina/mL containing 107 CFU/
mL of Candida parapsilosis. Forty-eight hour later the catheters were
filled with antifungal solution for further 48 hour. Treatment groups:
Control L-AmB 5 mg/mL, MICA 10 mg/mL, Ethanol 20%, Ethanol
40%, MICA solved in Eth 20% and MICA solved in Eth 40%. At the
end of the treatment period animals were sacrificed and CI was assessed
using sonication culture techniques. The total number of CFU
recovered from each catheter tip was recorded. Differences in %
negative catheters and log CFU were analyzed using Fisher and Mann-
Whitney tests; samples with no growth were assumed to be log = 0.3. A
p < 0.05 was considered significant. Results: Conclusions: Ethanol at 40% increases the efficacy of Micafungin in the treatment of Experimental Candida parapsilosis Catheter Infection Using the Antifungal-Lock Technique.

Abstract Number: NULL

Conference Year: 2012

Link to conference website: NULL

New link: NULL

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