Author:
Syun-ichi Urayama 1, Yuka Iwahashi 1, Shunsuke Masuo 1, Shusaku Kanematsu 1,
Hiromitsu Moriyama 2, Naoki Takaya 1, Nobuhiko Nomura 1, Norio Takeshita 1,
Masanori Toyofuku 1, Daisuke Hagiwara 1
Author address:
1 University of Tsukuba, Faculty of Life and Environmental Sciences, Tsukuba, Ibaraki, Japan;
2 Tokyo University of Agriculture and Technology, Laboratory of Molecular and Cellular Biology, Fuchu, Tokyo, Japan
Full conference title:
15th European Conference on Fungal Genetics 2020
Date: 20 June 2020
Abstract:
Extracellular membrane vesicles (eMV) are small, membrane-enclosed structures released from a cell into the surrounding environment (Toyofuku et al., 2018). eMV contains cargo molecules such as nucleic acids, proteins and chemical compounds, and affect diverse biological processes, including virulence, horizontal gene transfer and cell-to-cell communication. In the case of the fungi, recent studies demonstrated that fungi are able to produce biologically active EVs under culture and during infection (Souza et al., 2019). However, most studies have characterized EVs in yeast, while the release and characterization of these structures in filamentous fungi have been poorly explored. In this study, we analyzed the properties of eMV produced by filamentous fungi, Magnaporthe oryzae and Aspergillus oryzae.
Lipid particles appeared extracellular space during static liquid culture. When these lipid particles were purified and observed by TEM, vesicle structures were observed, indicating that these lipid particles are eMV. Proteome analysis of eMV fraction revealed that it contains plasma membrane-related proteins and secreted proteins. On agar media, production of eMV-like lipid particles on the surface of mycelia was observed by confocal laser microscope. These results suggested that M. oryzae and A. oryzae produce eMV during cultivation and eMV originate from plasma membrane. eMV is a kind of vector system which enables trans-cell wall transfer of biological components in filamentous fungi, and should be important to understanding the ecology and characteristics of filamentous fungi.
Link to conference website:
Link Conference abstract:
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