Airborne Fungi in A Hosp. Drug Dilution Ctr. Before and after the Cleaning Process

Author:

G. Link, A. Dias, L. B. Matter

Author address:

Univ.e Regional Integrada do Alto Uruguai e das Missões, Santo Angelo, RS, Brazil

Full conference title:

American Society for Microbiology (ASM) Microbe 2018

Date: 20 June 2020

Abstract:

Advances in surgical and drug therapy have prolonged patients’ lives but a population more vulnerable to opportunistic infections has emerged. In the hospital environment, where there are highly susceptible people, fungi represent a problem because their resistance and easy dissemination. Periodic cleaning process and monitoring of the air quality comprises an important strategy to preventing infections. In this context, the objective of this work was to verify the quality of the air in a hospital drug dilution center in Brazil, before and after the cleaning weekly procedure with 2% hypochlorite. The evaluation of the fungi in the air was achieved by exposing Petri dishes with Sabouraud agar according to the 1/1/1 scheme (for 1 hour, 1 m above the floor and 1 m away from walls or another dish). After incubation for 25 °C for 5 days, CFU were quantified, isolated and incubated again for macroscopic and microscopic analysis. Sampling showed a contamination of 57 CFU (average of 14 CFU/plate) and 50 CFU (average of 12 CFU/plate) before and after cleaning process, respectively. The main fungi found before cleaning were: Aspergillus sp., Penicillium sp., Curvularia sp., Trichophyton sp. and Fusarium sp. whereas after cleaning were found Trichophyton sp., Fusarium sp. and yeasts. This work revealed that the air quality of the drug dilution center was compromised even after the cleaning process and the efficiency of the cleaning process as well as the products used should be constantly evaluated to ensure the effectiveness of the procedure.

Abstract Number: AES01 (776)

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ASM microbe

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