Submitted by Aspergillus Administrator on 12 February 2013
This technique allows scientists to identify a wide range of microbial species quickly and easily – for example after air sampling. The technique relies on DNA sequences that have several specific properties (variable but not too variable and flanked by non-variable sequences!) which take some time to find. This new database lists a large number of suitable sequences that scientists can refer to if undertaking this type of research.
Fungal Ribosomal Internal Transcribed Spacer 1 Database– ITSoneDB is a comprehensive collection of the fungal ribosomal RNA Internal Transcribed Spacer 1 (ITS1) sequences aimed at supporting metagenomic surveys of fungal environmental communities. The sequences were extracted from Genbank (GB) and arranged on the NCBI taxonomy tree. ITS1 start and end boundaries were defined by GB annotations and/or designed by mapping Hidden Markov Model (HMM) profiles of flanking 18S and 5.8S ribosomal RNA coding genes on each sequence more…
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