Laboratory protocols

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Planar liquid chromatography
An Introduction to Planar Chromatography and Its Application to Natural Products Isolation
Fungal Molecular Biology
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
DNA extraction – miniprep from spores for PCR
Two new easy to use vectors for transformations (FGSC)
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Improved Vectors for Selecting Resistance to Hygromycin
A practical method for the preparation of total DNA from filamentous fungi
Growth and Storage
Care and feeding of Aspergillus (FGSC)
A list of Aspergillus mutations and how to deal with them
Protein methods
Investigation of In Vivo Protein Interactions in Aspergillus Spores
Multiplex Detection of Toxigenic Penicillium Species
Multiplex Detection of Mutations
General Molecular Biology
Media and Growth Conditions for Induction of Secondary Metabolite Production
Methods for DNA sequencing
Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species
Model systems: in vivo
Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis
DNA extraction
Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols
Fungal-Grade Reagents and Materials for Molecular Analysis
Identification of Mast Cells and Mast Cell Subpopulations
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Microarray Technologies in Fungal Diagnostics
A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection
Inducible Gene Expression Systems for Plants
Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi
Assaying mycotoxins
Determination of Aflatoxins and Zearalenone in Different Culture Media
Susceptibility testing
Screening for Antibacterial, Antifungal, and Anti quorum Sensing Activity