Laboratory protocols

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Detection in clinical samples
Fungal Diagnostics: Review of Commercially Available Methods
Protocol for homogenisation of CF sputum samples
Hyperbranching Rolling Circle Amplification, An Improved Protocol for Discriminating Between Closely Related Fungal Species
Cell Component Extraction
Transport Assays in Aspergillus nidulans
Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans
Fungal Molecular Biology
DNA Fingerprinting Candida Species
Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host (Aspergillus oryzae)
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Assaying mycotoxins
Chromatographic Method for AlternariaToxins in Apple Juice
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Analysis of Multiple Mycotoxins in Food
Simultaneous Determination of Aflatoxins B1, B2, G1, and G2 in Foods and Feed Materials
PCR
Real-Time Quantitative PCR, Pathogen Detection and MIQE
whole-cell screening
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Sampling Aspergillus spores
How to validate an automated colony counter
Protein methods
Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers (xylanase from Aspergillus niger)
Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides
Cell Wall Fractionation for Yeast and Fungal Proteomics
Model systems: in vitro
Antifungal Combinations
Species/Strain identification
Targeting Conserved Genes in Aspergillus Species
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Additional methods & reference
Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry
Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
Aflatoxins
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus

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