Laboratory protocols

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General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Protein methods
Investigation of In Vivo Protein Interactions in Aspergillus Spores
Growth and Storage
Care and feeding of Aspergillus (FGSC)
A list of Aspergillus mutations and how to deal with them
PCR
Multiplex Detection of Toxigenic Penicillium Species
Multiplex Detection of Mutations
Model systems: in vivo
Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis
Fungal Molecular Biology
Northern blot
Pathogen Gene Expression Profiling During Infection Using a Nanostring nCounter Platform
Two new easy to use vectors for transformations (FGSC)
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
A practical method for the preparation of total DNA from filamentous fungi
Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
Improved Vectors for Selecting Resistance to Hygromycin
DNA extraction
Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols
Fungal-Grade Reagents and Materials for Molecular Analysis
Histology
Identification of Mast Cells and Mast Cell Subpopulations
Assaying mycotoxins
Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of 5 azole antifungals and 1 active metabolite
Determination of Aflatoxins and Zearalenone in Different Culture Media
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Aflatoxins
A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection
Assaying antifungal levels
Voriconazole II Assay
Genetics
Inducible Gene Expression Systems for Plants
Susceptibility testing
Screening for Antibacterial, Antifungal, and Anti quorum Sensing Activity

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