Laboratory protocols

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Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans
Transport Assays in Aspergillus nidulans
Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans
Fungal Molecular Biology
High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
Species/Strain identification
Culture-Based Techniques
PCR
Real-Time Quantitative PCR, Pathogen Detection and MIQE
Targeting Aflatoxin Biosynthetic Genes
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Model systems: in vivo
Mouse Model of Invasive Fungal Infection
Application of Humanized Mice in Immunological Research
Model systems: in vitro
Antifungal Combinations
Protein methods
Cell Wall Fractionation for Yeast and Fungal Proteomics
Sampling Aspergillus spores
How to validate an automated colony counter
Microscopy
Preparation of samples for scanning electron microscopy
RT-qPCR
Targeting Other Mycotoxin Biosynthetic Genes
Secondary metabolites
The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
Growth and Storage
Fungal Isolation and Enumeration in Foods
Aflatoxins
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus
Methods for DNA sequencing
Library Preparation and Data Analysis Packages for Rapid Genome Sequencing
Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris

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