Laboratory protocols

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Fungal Molecular Biology
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
Signature-Tagged Mutagenesis
RNA extraction: large scale prep
Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification
PCR from spores
RNA extraction: Isolation of mRNA
Gene Transfer by Electroporation of Filamentous Fungi
Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR
Isolation of RNA from yeast
Protein methods
Protein Extraction from Fungi
Targeted Gene Disruption in Koji Mold Aspergillus oryzae
Differentiation of Fungi Using Hybridization Probes on the LightCycler®
Growth and Storage
Safe Dispatch and Transport of Biological Material
Model systems: in vivo
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
Detection in clinical samples
Protocol for homogenisation of CF sputum samples
Post Antifungal Effects (PAFE)
Postantifungal Effect Methods
Measurement of Aflatoxins Using Capillary Electrophoresis
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
Susceptibility testing
In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods
Natural Products and Antifungal Drug Discovery
Assaying antifungal levels
Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Posaconazole Antifungal Drug Level
Multi-Copy Genetic Screen in Aspergillus nidulans
Host-Derived Biomarkers for Risk Assessment of Invasive Fungal Diseases
Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans