Laboratory protocols

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Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
Protein methods
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Protein Extraction from Fungi
Assaying mycotoxins
Immunochemical Method for Ochratoxin A
Determination of optimum growth conditions for gliotoxin production by Aspergillus fumigatus and development of a novel method for gliotoxin detection.
Enzyme synthesis
Enzymatic Synthesis of α-Butylglucoside in a Biphasic Butanol-Water System Using the α-Transglucosidase from Aspergillus niger
Fungal Molecular Biology
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
Three Decades of Fungal Transformation
Susceptibility testing
EUCAST: MIC distributions and ECOFFs
Susceptibility testing of yeasts
EUCAST: Susceptibility testing in moulds
EUCAST: QC Tables for antifungal susceptibility testing
In situ hydridization
Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization
In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Secondary metabolites
Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi
Species/Strain identification
Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex® xMAP Technology
PCR-RFLP for Aspergillus Species
Assaying antifungal levels
Simple HPLC Method for the Determination of Caspofungin in Human Plasma
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
HPLC assay method for determining voriconazole levels in serum or plasma
Model systems: in vivo
Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Planar liquid chromatography
Isolation by Planar Chromatography
Additional methods & reference
Immunoinformatics as a Tool for New Antifungal Vaccines

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