Laboratory protocols

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Fungal Molecular Biology
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Determination of Plasmid Copy Number in Yeast
Protoplast Transformation of Filamentous Fungi
Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host (Aspergillus oryzae)
Cell Component Extraction
Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans
Growth and Storage
Sample Preparation Procedure for Cellular Fungi
Protein methods
Enzyme Immobilization Using Chitosan-Xanthan Complexes (including Aspergillus sojae protease type XIX)
Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers (xylanase from Aspergillus niger)
Additional methods & reference
In Vitro and Ex Vivo Assays of Virulence in Candida albicans
Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry
PCR
Multiplex Detection of Aspergillus Species (real-time PCR)
Aflatoxins
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus
Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
Assaying mycotoxins
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
Chromatographic Method for AlternariaToxins in Apple Juice
Analysis of Multiple Mycotoxins in Food
Detection in clinical samples
Fungal Diagnostics: Review of Commercially Available Methods
Enzyme synthesis
Enzymatic Synthesis of α-Butylglucoside in a Biphasic Butanol-Water System Using the α-Transglucosidase from Aspergillus niger
Susceptibility testing
EUCAST: MIC distributions and ECOFFs
Susceptibility testing of yeasts
whole-cell screening
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Species/Strain identification
Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex® xMAP Technology
Assaying antifungal levels
Simple HPLC Method for the Determination of Caspofungin in Human Plasma
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora

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