Laboratory protocols

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Model systems: in vivo
Mouse Model of Invasive Fungal Infection
Application of Humanized Mice in Immunological Research
Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
Fungal Molecular Biology
RNA extraction: large scale prep
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
PCR from spores
RNA extraction: Isolation of mRNA
Isolation of RNA from yeast
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
Differentiation of Fungi Using Hybridization Probes on the LightCycler®
Secondary metabolites
Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
Preparation of samples for scanning electron microscopy
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
HPLC assay method for determining voriconazole levels in serum or plasma
Measurement of Aflatoxins Using Capillary Electrophoresis
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
Targeting Other Mycotoxin Biosynthetic Genes
Growth and Storage
Fungal Isolation and Enumeration in Foods
Multi-Copy Genetic Screen in Aspergillus nidulans
Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans
Protein methods
Protein Extraction from Fungi
Investigation of In Vivo Protein Interactions in Aspergillus Spores
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris