Laboratory protocols

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Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi
Preparation of samples for scanning electron microscopy
Model systems: in vivo
Mouse Model of Invasive Fungal Infection
Application of Humanized Mice in Immunological Research
Targeting Aflatoxin Biosynthetic Genes
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus
Protocol overviews
Antifungal Pharmacokinetics and Pharmacodynamics
Video Protocols at the FGSC
Harvesting conidia from a 10 day old culture
Detection in clinical samples
Protocol for homogenisation of CF sputum samples
Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
Fungal Molecular Biology
Chromatin Immunoprecipitation Analysis in Filamentous Fungi
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
Protoplast Transformation of Filamentous Fungi
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
Additional methods & reference
In Vitro and Ex Vivo Assays of Virulence in Candida albicans
Growth and Storage
Vogel's Minimal Medium
FGSC culture preservation methods
Fungal Isolation and Enumeration in Foods
Media for culture of Aspergillus nidulans (FGSC)
Assaying mycotoxins
Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
Targeting Other Mycotoxin Biosynthetic Genes
Secondary metabolites
The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora