Laboratory protocols

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Model systems: in vivo
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Protein methods
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Isolation and Enrichment of Secreted Proteins from Filamentous Fungi
Species/Strain identification
PCR-RFLP for Aspergillus Species
Culture-Based Techniques
More protocol collections
Fungal Genetics Stock Centre
Cold Spring Harbour Protocols
Fungal Molecular Biology
Growing Fungi for DNA Extraction (FGSC)
Three Decades of Fungal Transformation
High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains
Overview of Invasive Fungal Infections
Assaying antifungal levels
Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
In situ hydridization
Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization
In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Video Protocols at the FGSC
Making a transfer
Making a rack of slants
Making a silica gel stock
Sealing an ampoule
Planar liquid chromatography
An Introduction to Planar Chromatography and Its Application to Natural Products Isolation
Additional methods & reference
Immunoinformatics as a Tool for New Antifungal Vaccines
Methods for DNA sequencing
Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species
Assaying mycotoxins
Immunochemical Method for Cyclopiazonic Acid
Protocol overviews
Overview of invasive fungal infections.
Microarray Technologies in Fungal Diagnostics