Laboratory protocols

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Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue
Protein methods
Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides
Secondary metabolites
Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi
Mycotoxins: A Fungal Genomics Perspective
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples
HPLC assay method for determining voriconazole levels in serum or plasma
ELISA for Aspergillus fumigatus in dust (CDC method)
Susceptibility testing
EUCAST: Susceptibility testing in moulds
EUCAST: QC Tables for antifungal susceptibility testing
Assaying antifungal levels
Simple HPLC Method for the Determination of Caspofungin in Human Plasma
Species/Strain identification
Identification of Fungal Pathogens in Tissue Samples from Patients with Proven Invasive Infection by Fluorescence In Situ Hybridization
PCR ITS-RFLP for Penicillium Species and Other Genera
More protocol collections
whole-cell screening
Susceptibility Testing Methods of Antifungal Agents
Planar liquid chromatography
Isolation by Planar Chromatography
Assaying mycotoxins
Determination of optimum growth conditions for gliotoxin production by Aspergillus fumigatus and development of a novel method for gliotoxin detection.
Fungal Molecular Biology
Fungal ABC Transporter Deletion and Localization Analysis (Penicillium chrysogenum)
SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption
Northern blot
General Molecular Biology
Adjuvant Properties of CpG Oligonucleotides in Primates
Additional methods & reference
ISHAM ITS Database
Clinical Breakpoints
EUCAST: Rationale documents on antifungal agents
Preparation of amphotericin paste
Protocol for aseptic preparation of amphotericin B paste for instillation into pulmonary cavity to treat inoperable aspergilloma
The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites