Laboratory protocols

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Growth and Storage
A list of Aspergillus mutations and how to deal with them
Fungal Molecular Biology
Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans
Two new easy to use vectors for transformations (FGSC)
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Protoplast Transformation of Filamentous Fungi
Improved Vectors for Selecting Resistance to Hygromycin
A practical method for the preparation of total DNA from filamentous fungi
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
Preparation of chromosomal DNA in agarose plugs
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
DNA extraction
Isolation of Nucleic Acids for Fungal Diagnosis
General Molecular Biology
Media and Growth Conditions for Induction of Secondary Metabolite Production
Additional methods & reference
In Vitro and Ex Vivo Assays of Virulence in Candida albicans
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Assaying mycotoxins
Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
Species/Strain identification
Molecular Detection of Resistance to Azole Components
whole-cell screening
Screening of a Marine Algal Extract for Antifungal Activities
Detection in clinical samples
Protocol for homogenisation of CF sputum samples
Immunological Identification of Fungal Species
RT-qPCR
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
Genetics
Inducible Gene Expression Systems for Plants
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
Protein methods
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Protocol overviews
Three Decades of Fungal Transformation: Key Concepts and Applications
Biofilm
In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development

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