Laboratory protocols

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Fungal Molecular Biology
Pathogen Gene Expression Profiling During Infection Using a Nanostring nCounter Platform
Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
Three Decades of Fungal Transformation
High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains
Overview of Invasive Fungal Infections
Model systems: in vivo
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Mouse Model of Invasive Fungal Infection
Video Protocols at the FGSC
Making a transfer
Making a rack of slants
Making a silica gel stock
Sealing an ampoule
Assaying mycotoxins
Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of 5 azole antifungals and 1 active metabolite
Immunochemical Method for Ochratoxin A
Immunochemical Method for Cyclopiazonic Acid
In situ hydridization
In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
More protocol collections
Cold Spring Harbour Protocols
Additional methods & reference
Immunoinformatics as a Tool for New Antifungal Vaccines
Assaying antifungal levels
Voriconazole II Assay
Protocol overviews
Overview of invasive fungal infections.
Antifungal Pharmacokinetics and Pharmacodynamics
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
HPLC assay method for determining voriconazole levels in serum or plasma
Protein methods
Isolation and Enrichment of Secreted Proteins from Filamentous Fungi
Secondary metabolites
Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
Species/Strain identification
Culture-Based Techniques