Laboratory protocols

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Video Protocols at the FGSC
Making a rack of slants
Making a silica gel stock
Sealing an ampoule
Harvesting conidia from a 10 day old culture
Fungal Molecular Biology
Cell Fusion in the Filamentous Fungus, Neurospora crassa
High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains
DNA Fingerprinting Candida Species
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
Overview of Invasive Fungal Infections
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
PCR
Targeting Aflatoxin Biosynthetic Genes
Assaying antifungal levels
Bioassay for Determining Voriconazole Levels in Blood
Model systems: in vivo
Mouse Model of Invasive Fungal Infection
Application of Humanized Mice in Immunological Research
Protocol overviews
Overview of invasive fungal infections.
Antifungal Pharmacokinetics and Pharmacodynamics
Protein methods
Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Cell Wall Fractionation for Yeast and Fungal Proteomics
Microscopy
Preparation of samples for scanning electron microscopy
Secondary metabolites
The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
RT-qPCR
Targeting Other Mycotoxin Biosynthetic Genes
Growth and Storage
Vogel's Minimal Medium
Fungal Isolation and Enumeration in Foods
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris

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