Laboratory protocols

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The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites
General Molecular Biology
Adjuvant Properties of CpG Oligonucleotides in Primates
DNA extraction
Isolation of Nucleic Acids for Fungal Diagnosis
Susceptibility testing
EUCAST Technical Note on Aspergillus and amphotericin B, itraconazole, and posaconazole
EUCAST: Veterinary Committee on Antimicrobial Susceptibility Testing (VetCAST)
Preparation of amphotericin paste
Bedside preparation of amphotericin B paste
Fungal Molecular Biology
A novel method of growing fungi for DNA extraction
Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans
DNA extraction – miniprep from spores for PCR
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
Total DNA Isolation from Filamentous Fungi (FGSC)
Secondary metabolites
Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
Genetics
Evaluation of the Disease Liability of CFTR Variants
whole-cell screening
Screening of a Marine Algal Extract for Antifungal Activities
Histology
Histology: Guide to special stains
Model systems: in vivo
Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
Species/Strain identification
Molecular Detection of Resistance to Azole Components
PCR
Arbitrarily Primed PCR Methods for Studying Bacterial Diseases
Detection in clinical samples
Immunological Identification of Fungal Species
Development and Evaluation of a Calibrator Material for Nucleic Acid-Based Assays for Diagnosing Aspergillosis
Veterinary
Isolation of Rumen Fungi
Protein methods
Protein Extraction from Fungi
RT-qPCR
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
Assaying antifungal levels
Bioassay for Determining Voriconazole Levels in Blood
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