Laboratory protocols

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Video Protocols at the FGSC
Making a silica gel stock
Sealing an ampoule
Harvesting conidia from a 10 day old culture
Protein methods
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Isolation and Enrichment of Secreted Proteins from Filamentous Fungi
Model systems: in vivo
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Fungal Molecular Biology
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
Overview of Invasive Fungal Infections
Three Decades of Fungal Transformation
In situ hydridization
Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization
In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Assaying antifungal levels
Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Species/Strain identification
PCR-RFLP for Aspergillus Species
Culture-Based Techniques
Protocol overviews
Antifungal Pharmacokinetics and Pharmacodynamics
Planar liquid chromatography
An Introduction to Planar Chromatography and Its Application to Natural Products Isolation
Detection in clinical samples
Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
Growth and Storage
Vogel's Minimal Medium
FGSC culture preservation methods
Methods for DNA sequencing
Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species
Additional methods & reference
Immunoinformatics as a Tool for New Antifungal Vaccines
PCR
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
Microarray Technologies in Fungal Diagnostics
Assaying mycotoxins
Immunochemical Method for Cyclopiazonic Acid

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