Laboratory protocols

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Species/Strain identification
PCR ITS-RFLP for Penicillium Species and Other Genera
Molecular Detection of Resistance to Azole Components
Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex® xMAP Technology
Fungal Molecular Biology
DNA extraction: miniprep
Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans
A novel method of growing fungi for DNA extraction
DNA extraction – miniprep from spores for PCR
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
Protein methods
Protein Extraction from Fungi
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Aflatoxins
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus
Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
DNA extraction
Isolation of Nucleic Acids for Fungal Diagnosis
Model systems: in vivo
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
Enzyme synthesis
Enzymatic Synthesis of α-Butylglucoside in a Biphasic Butanol-Water System Using the α-Transglucosidase from Aspergillus niger
Susceptibility testing
EUCAST: MIC distributions and ECOFFs
Susceptibility testing of yeasts
EUCAST: Susceptibility testing in moulds
whole-cell screening
Screening of a Marine Algal Extract for Antifungal Activities
Assaying antifungal levels
Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Simple HPLC Method for the Determination of Caspofungin in Human Plasma
RT-qPCR
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
Detection in clinical samples
Immunological Identification of Fungal Species
Planar liquid chromatography
An Introduction to Planar Chromatography and Its Application to Natural Products Isolation
Secondary metabolites
Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi

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