Laboratory protocols

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Detection in clinical samples
Fungal Diagnostics: Review of Commercially Available Methods
Protocol overviews
Three Decades of Fungal Transformation: Key Concepts and Applications
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation
Fungal Molecular Biology
SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
Preparation of chromosomal DNA in agarose plugs
Northern blot
Lambda IIBargem7B
RNA extraction: miniprep
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
Pathogen Gene Expression Profiling During Infection Using a Nanostring nCounter Platform
DNA extraction: miniprep
RNA extraction: large scale prep
Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host (Aspergillus oryzae)
Additional methods & reference
ISHAM ITS Database
Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry
Assaying mycotoxins
Chromatographic Method for AlternariaToxins in Apple Juice
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Analysis of Multiple Mycotoxins in Food
Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of 5 azole antifungals and 1 active metabolite
whole-cell screening
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Protein methods
Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers (xylanase from Aspergillus niger)
Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides
Species/Strain identification
Targeting Conserved Genes in Aspergillus Species
Model systems: in vivo
Animal Models to Evaluate Anti-infective Pharmacodynamics

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