Laboratory protocols

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Preparation of amphotericin paste
Bedside preparation of amphotericin B paste
Protein methods
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Protein Extraction from Fungi
Isolation and Enrichment of Secreted Proteins from Filamentous Fungi
PCR
Arbitrarily Primed PCR Methods for Studying Bacterial Diseases
Fungal Molecular Biology
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
Total DNA Isolation from Filamentous Fungi (FGSC)
Growing Fungi for DNA Extraction (FGSC)
Three Decades of Fungal Transformation
Assaying mycotoxins
Immunochemical Method for Ochratoxin A
Immunochemical Method for Cyclopiazonic Acid
Susceptibility testing
EUCAST: Veterinary Committee on Antimicrobial Susceptibility Testing (VetCAST)
Histology
Histology: Guide to special stains
Veterinary
Isolation of Rumen Fungi
In situ hydridization
Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization
In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Species/Strain identification
PCR-RFLP for Aspergillus Species
Secondary metabolites
Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
Detection in clinical samples
Development and Evaluation of a Calibrator Material for Nucleic Acid-Based Assays for Diagnosing Aspergillosis
Model systems: in vivo
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
More protocol collections
Current Protocols
Fungal Genetics Stock Centre
Additional methods & reference
Immunoinformatics as a Tool for New Antifungal Vaccines
Video Protocols at the FGSC
Making a transfer

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