Laboratory protocols

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Fungal Molecular Biology
DNA Fingerprinting Candida Species
Total DNA Isolation from Filamentous Fungi (FGSC)
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
Growing Fungi for DNA Extraction (FGSC)
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
More protocol collections
Current Protocols
Fungal Genetics Stock Centre
Cold Spring Harbour Protocols
Microscopy
Preparation of samples for scanning electron microscopy
Protein methods
Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Cell Wall Fractionation for Yeast and Fungal Proteomics
Investigation of In Vivo Protein Interactions in Aspergillus Spores
RT-qPCR
Targeting Other Mycotoxin Biosynthetic Genes
Detection in clinical samples
Protocol for homogenisation of CF sputum samples
Secondary metabolites
The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Model systems: in vivo
Application of Humanized Mice in Immunological Research
Induction of Allergic Airway Disease Using House Dust Mite Allergen
Video Protocols at the FGSC
Making a transfer
Making a rack of slants
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
Growth and Storage
Fungal Isolation and Enumeration in Foods
Protocol overviews
Overview of invasive fungal infections.

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