Laboratory protocols

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Model systems: in vitro
Antifungal Combinations
PCR
Multiplex Detection of Aspergillus Species (real-time PCR)
Sampling Aspergillus spores
How to validate an automated colony counter
More protocol collections
AspGD
Assaying mycotoxins
Chromatographic Method for AlternariaToxins in Apple Juice
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Analysis of Multiple Mycotoxins in Food
Fungal Molecular Biology
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
SYBR safeTM efficiently replaces ethidium bromide in Aspergillus fumigatus gene disruption
Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host (Aspergillus oryzae)
Northern blot
Detection in clinical samples
Fungal Diagnostics: Review of Commercially Available Methods
ELISA for Aspergillus fumigatus in dust (CDC method)
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
HPLC assay method for determining voriconazole levels in serum or plasma
Cell Component Extraction
Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans
Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
Protein methods
Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers (xylanase from Aspergillus niger)
Aflatoxins
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus
whole-cell screening
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Susceptibility testing
EUCAST: MIC distributions and ECOFFs
Susceptibility testing of yeasts
Additional methods & reference
Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry
Enzyme synthesis
Enzymatic Synthesis of α-Butylglucoside in a Biphasic Butanol-Water System Using the α-Transglucosidase from Aspergillus niger
Species/Strain identification
Targeting Conserved Genes in Aspergillus Species

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