Laboratory protocols

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Video Protocols at the FGSC
Sealing an ampoule
Harvesting conidia from a 10 day old culture
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
Secondary metabolites
Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays
Fungal Molecular Biology
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
Overview of Invasive Fungal Infections
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
Growth and Storage
Fungal Isolation and Enumeration in Foods
Vogel's Minimal Medium
FGSC culture preservation methods
Media for culture of Aspergillus nidulans (FGSC)
Model systems: in vivo
Induction of Allergic Airway Disease Using House Dust Mite Allergen
Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
Protein methods
Investigation of In Vivo Protein Interactions in Aspergillus Spores
Protein Extraction from Fungi
Protocol overviews
Antifungal Pharmacokinetics and Pharmacodynamics
PCR
Multiplex Detection of Toxigenic Penicillium Species
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
DNA extraction
Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols
Fungal-Grade Reagents and Materials for Molecular Analysis
Detection in clinical samples
Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
Histology
Identification of Mast Cells and Mast Cell Subpopulations
Aflatoxins
A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection

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