Laboratory protocols

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Protocol overviews
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation
Model systems: in vivo
Animal Models to Evaluate Anti-infective Pharmacodynamics
Fungal Molecular Biology
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
RNA extraction: large scale prep
PCR from spores
RNA extraction: Isolation of mRNA
Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens- Mediated Transformation
Isolation of RNA from yeast
Broad-Spectrum Molecular Detection of Fungal Nucleic Acids by PCR-Based Amplification Techniques
Cell Fusion in the Filamentous Fungus, Neurospora crassa
Species/Strain identification
Molecular Detection of Resistance to Azole Components
PCR-RFLP for Aspergillus Species
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
whole-cell screening
Screening of a Marine Algal Extract for Antifungal Activities
Assaying antifungal levels
Bioassay for Determining Voriconazole Levels in Blood
PCR
Differentiation of Fungi Using Hybridization Probes on the LightCycler®
RT-qPCR
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
Biofilm
In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
Detection in clinical samples
Immunological Identification of Fungal Species
Aflatoxins
Measurement of Aflatoxins Using Capillary Electrophoresis
Protein methods
Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gel
Genetics
Multi-Copy Genetic Screen in Aspergillus nidulans
Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans
In situ hydridization
Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization

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