Laboratory protocols

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Targeting Aflatoxin Biosynthetic Genes
Arbitrarily Primed PCR Methods for Studying Bacterial Diseases
Planar liquid chromatography
Isolation by Planar Chromatography
Fungal Molecular Biology
Protoplast Transformation of Filamentous Fungi
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens- Mediated Transformation
Susceptibility testing
EUCAST: QC Tables for antifungal susceptibility testing
EUCAST Technical Note on Aspergillus and amphotericin B, itraconazole, and posaconazole
EUCAST: Veterinary Committee on Antimicrobial Susceptibility Testing (VetCAST)
Preparation of samples for scanning electron microscopy
Assaying mycotoxins
Determination of optimum growth conditions for gliotoxin production by Aspergillus fumigatus and development of a novel method for gliotoxin detection.
General Molecular Biology
Adjuvant Properties of CpG Oligonucleotides in Primates
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
Clinical Breakpoints
EUCAST: Rationale documents on antifungal agents
Targeting Other Mycotoxin Biosynthetic Genes
Preparation of amphotericin paste
Protocol for aseptic preparation of amphotericin B paste for instillation into pulmonary cavity to treat inoperable aspergilloma
Bedside preparation of amphotericin B paste
Secondary metabolites
The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
Model systems: in vivo
Application of Humanized Mice in Immunological Research
Growth and Storage
Fungal Isolation and Enumeration in Foods
Evaluation of the Disease Liability of CFTR Variants
In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
Protein methods
Investigation of In Vivo Protein Interactions in Aspergillus Spores