Laboratory protocols

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Fungal Molecular Biology
Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host (Aspergillus oryzae)
Chromatin Immunoprecipitation Analysis in Filamentous Fungi
Two new easy to use vectors for transformations (FGSC)
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Improved Vectors for Selecting Resistance to Hygromycin
Fungal ABC Transporter Deletion and Localization Analysis (Penicillium chrysogenum)
A practical method for the preparation of total DNA from filamentous fungi
Protoplast Transformation of Filamentous Fungi
Species/Strain identification
Targeting Conserved Genes in Aspergillus Species
Identification of Fungal Pathogens in Tissue Samples from Patients with Proven Invasive Infection by Fluorescence In Situ Hybridization
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Additional methods & reference
Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid-Phase Microextraction-Gas Chromatography/Mass Spectrometry
In Vitro and Ex Vivo Assays of Virulence in Candida albicans
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Growth and Storage
A list of Aspergillus mutations and how to deal with them
Assaying mycotoxins
Simultaneous Determination of Aflatoxins B1, B2, G1, and G2 in Foods and Feed Materials
Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
Protein methods
Screening of Microorganisms for Enzymatic Biosynthesis of Nondigestible Oligosaccharides
Detection in clinical samples
Hyperbranching Rolling Circle Amplification, An Improved Protocol for Discriminating Between Closely Related Fungal Species
Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples
whole-cell screening
Susceptibility Testing Methods of Antifungal Agents
Secondary metabolites
Mycotoxins: A Fungal Genomics Perspective
Genetics
Inducible Gene Expression Systems for Plants
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora

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