Laboratory protocols

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Model systems: in vivo
Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
Biofilm
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
Histology
Identification of Mast Cells and Mast Cell Subpopulations
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
HPLC assay method for determining voriconazole levels in serum or plasma
Fungal Molecular Biology
Isolation of RNA from yeast
Signature-Tagged Mutagenesis
Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification
DNA extraction
Fungal-Grade Reagents and Materials for Molecular Analysis
Aflatoxins
A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection
Measurement of Aflatoxins Using Capillary Electrophoresis
Protein methods
Protein Extraction from Fungi
Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans
Transport Assays in Aspergillus nidulans
PCR
Multiplex Detection of Mutations
Real-Time Quantitative PCR, Pathogen Detection and MIQE
Assaying mycotoxins
Determination of Aflatoxins and Zearalenone in Different Culture Media
Susceptibility testing
Screening for Antibacterial, Antifungal, and Anti quorum Sensing Activity
Genetics
Multi-Copy Genetic Screen in Aspergillus nidulans
Additional methods & reference
ISHAM ITS Database
Biotyping
Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity
Model systems: in vitro
Antifungal Combinations
Assaying antifungal levels
Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Sampling Aspergillus spores
How to validate an automated colony counter

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