Laboratory protocols

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Targeting Aflatoxin Biosynthetic Genes
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus
Microarray Technologies in Fungal Diagnostics
Growth and Storage
FGSC culture preservation methods
Media for culture of Aspergillus nidulans (FGSC)
Care and feeding of Aspergillus (FGSC)
Fungal Isolation and Enumeration in Foods
A list of Aspergillus mutations and how to deal with them
Model systems: in vivo
Mouse Model of Invasive Fungal Infection
Application of Humanized Mice in Immunological Research
Fungal Molecular Biology
Identification of Ochratoxin A-Producing Black Aspergilli from Grapes Using Loop-Mediated Isothermal Amplification (LAMP) Assays
Chromatin Immunoprecipitation Analysis in Filamentous Fungi
Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
Methods to Detect Apoptotic-Like Cell Death in Filamentous Fungi
Preparation of samples for scanning electron microscopy
Secondary metabolites
The “FERMEX” Method for Metabolite-Enriched Fungal Extracts
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Targeting Other Mycotoxin Biosynthetic Genes
Additional methods & reference
In Vitro and Ex Vivo Assays of Virulence in Candida albicans
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Enzyme synthesis
Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris
Protein methods
Investigation of In Vivo Protein Interactions in Aspergillus Spores