Laboratory protocols

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Model systems: in vivo
Murine Model of Invasive Aspergillosis
Assaying mycotoxins
Development and validation of a liquid chromatography-tandem mass spectrometry assay for the simultaneous quantitation of 5 azole antifungals and 1 active metabolite
Immunochemical Method for Ochratoxin A
Chromatographic Method for AlternariaToxins in Apple Juice
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Analysis of Multiple Mycotoxins in Food
Fungal Molecular Biology
Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis
Pathogen Gene Expression Profiling During Infection Using a Nanostring nCounter Platform
Development of Impala-Based Transposon Systems for Gene Tagging in Filamentous Fungi
Determination of Plasmid Copy Number in Yeast
Growth and Storage
Sample Preparation Procedure for Cellular Fungi
Cell Component Extraction
Determination of D-galactofuranose Content of Galactomannoproteins in Aspergillus nidulans
Protein methods
High-Throughput Screening of Dye-Ligands for Chromatography (on dehydrogenases from Aspergillus nidulans)
Enzyme Immobilization Using Chitosan-Xanthan Complexes (including Aspergillus sojae protease type XIX)
PCR
Multiplex Detection of Aspergillus Species (real-time PCR)
Assaying antifungal levels
Voriconazole II Assay
Aflatoxins
Purification of a Vesicle–Vacuole (V) Fraction from Aspergillus
Clinical Breakpoints
EUCAST: Clinical Breakpoint table for Candida spp and Aspergillus spp
Detection in clinical samples
Fungal Diagnostics: Review of Commercially Available Methods
Protocol for homogenisation of CF sputum samples
Enzyme synthesis
Enzymatic Synthesis of α-Butylglucoside in a Biphasic Butanol-Water System Using the α-Transglucosidase from Aspergillus niger
Susceptibility testing
EUCAST: MIC distributions and ECOFFs
whole-cell screening
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Species/Strain identification
Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by the Luminex® xMAP Technology
Secondary metabolites
Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays

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