Laboratory protocols

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Model systems: in vivo
Induction of Allergic Airway Disease Using House Dust Mite Allergen
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis
Animal Models to Evaluate Anti-infective Pharmacodynamics
Fungal Molecular Biology
Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
Preparation of chromosomal DNA in agarose plugs
Lambda IIBargem7B
RNA extraction: miniprep
A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction
RNA extraction: large scale prep
Protocol overviews
Three Decades of Fungal Transformation: Key Concepts and Applications
Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation
Protein methods
Investigation of In Vivo Protein Interactions in Aspergillus Spores
PCR
Multiplex Detection of Toxigenic Penicillium Species
Multiplex Detection of Mutations
Differentiation of Fungi Using Hybridization Probes on the LightCycler®
DNA extraction
Bacterial and Fungal DNA Extraction from Blood Samples: Manual Protocols
Fungal-Grade Reagents and Materials for Molecular Analysis
Assaying antifungal levels
Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Planar liquid chromatography
An Introduction to Planar Chromatography and Its Application to Natural Products Isolation
Histology
Identification of Mast Cells and Mast Cell Subpopulations
Aflatoxins
A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection
Methods for DNA sequencing
Using RNA-seq for Analysis of Differential Gene Expression in Fungal Species
Assaying mycotoxins
Determination of Aflatoxins and Zearalenone in Different Culture Media

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