Laboratory protocols

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Fungal Molecular Biology
Signature-Tagged Mutagenesis
Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification
DNA Fingerprinting Candida Species
Gene Transfer by Electroporation of Filamentous Fungi
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Expression Profiling of Fungal Genes During Arbuscular Mycorrhiza Symbiosis Establishment Using Direct Fluorescent In Situ RT-PCR
Two new easy to use vectors for transformations (FGSC)
A practical method for the preparation of total DNA from filamentous fungi
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Biotyping
Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity
Growth and Storage
Care and feeding of Aspergillus (FGSC)
Safe Dispatch and Transport of Biological Material
A list of Aspergillus mutations and how to deal with them
Protein methods
Targeted Gene Disruption in Koji Mold Aspergillus oryzae
Making a Protein Extract from Plant Pathogenic Fungi for Gel- and LC-Based Proteomics
Cell Wall Fractionation for Yeast and Fungal Proteomics
Susceptibility testing
In Vitro and In Vivo Antibacterial and Antifungal Screening of Natural Plant Products: Prospective Standardization of Basic Methods
Natural Products and Antifungal Drug Discovery
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
Post Antifungal Effects (PAFE)
Postantifungal Effect Methods
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Genetics
Host-Derived Biomarkers for Risk Assessment of Invasive Fungal Diseases
Inducible Gene Expression Systems for Plants
Assaying antifungal levels
Posaconazole Antifungal Drug Level

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