Laboratory protocols

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Model systems: in vivo
Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse
Experimental Infection of Rodent Mammals for Fungal Virulence Testing
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
Identification of Mast Cells and Mast Cell Subpopulations
Detection in clinical samples
LC-MS/MS assay method for determining voriconazole levels in serum or plasma
HPLC assay method for determining voriconazole levels in serum or plasma
Fungal Molecular Biology
Isolation of RNA from yeast
Signature-Tagged Mutagenesis
Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification
DNA extraction
Fungal-Grade Reagents and Materials for Molecular Analysis
A Confirmatory Method for Aflatoxin M1 Determination in Milk Based on Immunoaffinity Cleanup and High-Performance Liquid Chromatography with Fluorometric Detection
Measurement of Aflatoxins Using Capillary Electrophoresis
Protein methods
Protein Extraction from Fungi
Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans
Transport Assays in Aspergillus nidulans
Multiplex Detection of Mutations
Real-Time Quantitative PCR, Pathogen Detection and MIQE
Assaying mycotoxins
Determination of Aflatoxins and Zearalenone in Different Culture Media
Susceptibility testing
Screening for Antibacterial, Antifungal, and Anti quorum Sensing Activity
Multi-Copy Genetic Screen in Aspergillus nidulans
Additional methods & reference
ISHAM ITS Database
Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity
Model systems: in vitro
Antifungal Combinations
Assaying antifungal levels
Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Sampling Aspergillus spores
How to validate an automated colony counter