Laboratory protocols

Displaying 1 - 25 of 366
In situ hydridization
In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes
Assaying mycotoxins
Microarray-Based Immunoassay for Parallel Quantification of Multiple Mycotoxins in Oat
Immunochemical Method for Cyclopiazonic Acid
Model systems: in vivo
An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airway Disease
Mouse Model of Invasive Fungal Infection
Protocol overviews
Antifungal Pharmacokinetics and Pharmacodynamics
Video Protocols at the FGSC
Harvesting conidia from a 10 day old culture
Fungal Molecular Biology
Three Decades of Fungal Transformation
Protoplast Transformation of Filamentous Fungi
High-Throughput Construction of Gene Deletion Cassettes for Generation of Neurospora crassa Knockout Strains
Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens- Mediated Transformation
Additional methods & reference
Immunoinformatics as a Tool for New Antifungal Vaccines
Detection in clinical samples
Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification
Protocol for homogenisation of CF sputum samples
Growth and Storage
Vogel's Minimal Medium
FGSC culture preservation methods
Media for culture of Aspergillus nidulans (FGSC)
Care and feeding of Aspergillus (FGSC)
Protein methods
Isolation and Enrichment of Secreted Proteins from Filamentous Fungi
Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora
PCR
Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions
Highly Sensitive PCR-Based Detection Specific to Aspergillus flavus
Targeting Aflatoxin Biosynthetic Genes
Biofilm
In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development
Species/Strain identification
Culture-Based Techniques

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