Laboratory protocols

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Growth and Storage
Care and feeding of Aspergillus (FGSC)
A list of Aspergillus mutations and how to deal with them
Detection in clinical samples
Real-Time PCR-Based Identification of Bacterial and Fungal Pathogens from Blood Samples
ELISA for Aspergillus fumigatus in dust (CDC method)
Species/Strain identification
Identification of Fungal Pathogens in Tissue Samples from Patients with Proven Invasive Infection by Fluorescence In Situ Hybridization
PCR ITS-RFLP for Penicillium Species and Other Genera
Protein methods
A Multi-Step Chromatographic Strategy to Purify Three Fungal Endo-β-Glucanases
whole-cell screening
Susceptibility Testing Methods of Antifungal Agents
General Molecular Biology
Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry
Media and Growth Conditions for Induction of Secondary Metabolite Production
Fungal Molecular Biology
Fungal ABC Transporter Deletion and Localization Analysis (Penicillium chrysogenum)
Two new easy to use vectors for transformations (FGSC)
A rapid method for isolation of total nucleic acids from Aspergillus nidulans.
Improved Vectors for Selecting Resistance to Hygromycin
A practical method for the preparation of total DNA from filamentous fungi
Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans
A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites
Histology
Assessment of Immune Responses to Fungal Infections: Identification and Characterization of Immune Cells in the Infected Tissue
Secondary metabolites
Mycotoxins: A Fungal Genomics Perspective
Assaying antifungal levels
Bioassay for Determining Voriconazole Levels in Blood
The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites
More protocol collections
AspGD
Additional methods & reference
In Silico Systems Biology Approaches for the Identification of Antimicrobial Targets
DNA extraction
Isolation of Nucleic Acids for Fungal Diagnosis
Genetics
Inducible Gene Expression Systems for Plants

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