Laboratory protocols

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Protein methods
High-Throughput Screening of Dye-Ligands for Chromatography (on dehydrogenases from Aspergillus nidulans)
Enzyme Immobilization Using Chitosan-Xanthan Complexes (including Aspergillus sojae protease type XIX)
Cell Wall Fractionation for Yeast and Fungal Proteomics
Design of Smart Biocatalysts: Immobilization of Enzymes on Smart Polymers (xylanase from Aspergillus niger)
Biofilm
Bioluminescence Imaging of Fungal Biofilm Development in Live Animals
Establishing a Biofilm Co-culture of Pseudomonas and Aspergillus for Metabolite Extraction
Aflatoxins
Measurement of Aflatoxins Using Capillary Electrophoresis
Model systems: in vivo
Murine Model of Invasive Aspergillosis
Fungal Molecular Biology
Isolation of RNA from yeast
Determination of Plasmid Copy Number in Yeast
Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi
Growth and Storage
Sample Preparation Procedure for Cellular Fungi
Genetics
Multi-Copy Genetic Screen in Aspergillus nidulans
PCR
Multiplex Detection of Aspergillus Species (real-time PCR)
Real-Time Quantitative PCR, Pathogen Detection and MIQE
Cell Component Extraction
Purification of the GfsA-3x FLAG Protein Expressed in Aspergillus nidulans
Transport Assays in Aspergillus nidulans
Assaying mycotoxins
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Chromatographic Method for AlternariaToxins in Apple Juice
Detection in clinical samples
Fungal Diagnostics: Review of Commercially Available Methods
Protocol for homogenisation of CF sputum samples
Methods for DNA sequencing
Library Preparation and Data Analysis Packages for Rapid Genome Sequencing
Model systems: in vitro
Antifungal Combinations
whole-cell screening
A Fluorescence-Based High-Throughput Screening Assay to Identify Growth Inhibitors of the Pathogenic Fungus Aspergillus fumigatus
Sampling Aspergillus spores
How to validate an automated colony counter

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