Kinase end-labeling of DNA

ID: 52


General Molecular Biology

Prepared by:

Paul Bowyer


Typical 5′-kinase labeling reactions included the DNA to be labeled, [[gamma]]-32-P-dATP, T4 polynucleotide kinase, and buffer. After incubation at 37degC, reactions are heat inactivated by incubation at 80degC. Portions of the reactions are mixed with gel loading dye and loaded into a well of a polyacrylamide gel and electrophoresed.

Year prepared: 2009

Date uploaded: 2010-02-26 14:11:47


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