EUCAST Technical Note on the method for the determination of broth dilution minimum inhibitory concentrations of antifungal agents for conidia-forming moulds

ID: 27



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Antifungal susceptibility tests are performed on those fungi causing disease, especially if they belong to a species exhibiting resistance to commonly used antifungal agents. Antifungal susceptibility testing is also important for resistance surveillance, epidemiological studies and for comparing the in vitro activity of new and established agents.
Reference methods for antimicrobial susceptibility testing rely on incremental dilution of the antimicrobial agents to determine the minimum inhibitory concentrations (MICs) and are mainly used to establish the activity of a new agent, to confirm the susceptibility of microorganisms that yield equivocal results in routine tests, or to determine their susceptibility when routine tests are either unreliable or not readily available.
There is also a need for standardized methods for determining the in vitro susceptibilities of both new and established antifungal agents against
clinical isolates of filamentous fungi as there is an increasing number of agents to choose from for treating invasive mould disease, and resistance to antifungal agents in some species has been documented [1–9].
The Subcommittee on Antifungal Susceptibility Testing (AFST) of the European Committee for Antimicrobial Susceptibility Testing (EUCAST) has developed a broth dilution methodology for determining the antifungal susceptibility of conidia-forming moulds that cause clinically significant
invasive fungal disease. This technical note is based on the EUCAST method and the definitive document (E.DEF 9.1) is available in full on the EUCAST website at

Year prepared: 2008

Date uploaded: 2010-02-26 14:40:21

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