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Spore catches (from Cunningham DD, Microscopic Examination of Air, 1873):
Spore catches (from Cunningham DD, Microscopic Examination of Air, 1873):

Cunningham, D.D. 1873. Microscopic examination of air. Govt. of India Publication, Calcutta.

Sporetrap circa 1873
Sporetrap circa 1873

Cunningham, D.D. 1873. Microscopic examination of air. Govt. of India Publication, Calcutta.

KOH preparation of a respiratory fluid showing wide non-septate hyphae, typical of a Zygomycete. KOH preparation of a respiratory fluid showing wide non-septate hyphae, typical of a Zygomycete.
KOH preparation of a respiratory fluid showing wide non-septate hyphae, typical of a Zygomycete. KOH preparation of a respiratory fluid showing wide non-septate hyphae, typical of a Zygomycete.
BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with KOH BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with KOH
BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with KOH BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with KOH
BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with Blankophor BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with Blankophor
BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with Blankophor BAL specimen showing hyaline, septate hyphae consistent with Aspergillus, stained with Blankophor
Mucous plug examined by light microscopy with KOH Mucous plug examined by light microscopy with KOH, showing a network of hyaline branching hyphae typical of Aspergillus, from a patient with ABPA.
Mucous plug examined by light microscopy with KOH Mucous plug examined by light microscopy with KOH, showing a network of hyaline branching hyphae typical of Aspergillus, from a patient with ABPA.
Corneal scraping stained with lactophenol cotton blue Corneal scraping stained with lactophenol cotton blue showing beaded septate hyphae not typical of either Fusarium spp or Aspergillus spp, being more consistent with a dematiceous (ie brown coloured) fungus
Corneal scraping stained with lactophenol cotton blue Corneal scraping stained with lactophenol cotton blue showing beaded septate hyphae not typical of either Fusarium spp or Aspergillus spp, being more consistent with a dematiceous (ie brown coloured) fungus
Corneal scrape with lactophenol cotton blue Corneal scrape with lactophenol cotton blue shows separate hyphae with Fusarium spp or Aspergillus spp.
Corneal scrape with lactophenol cotton blue Corneal scrape with lactophenol cotton blue shows separate hyphae with Fusarium spp or Aspergillus spp.
Specimens taken from nails
Specimens taken from nails

The quality of the specimen taken is a major factor in success or otherwise of microscopy and culture. Having a specimen taken should be painless apart from occasional slight discomfort when subungual specimens are taken. The figure shows the appropriate sites from which nail specimens should be obtained.

Skin scraping showing filamentous hyphae with blankophor.
A filamentous fungus in the CSF of a patient with meningitis A filamentous fungus in the CSF of a patient with meningitis that grew Candida albicans in culture subsequently.
A filamentous fungus in the CSF of a patient with meningitis A filamentous fungus in the CSF of a patient with meningitis that grew Candida albicans in culture subsequently.
Transmission electron micrograph of a C. neoformans Transmission electron micrograph of a C. neoformans cell seen in CSF in an AIDS patients with remarkably little capsule present. These cells may be mistaken for lymphocytes.
Transmission electron micrograph of a C. neoformans Transmission electron micrograph of a C. neoformans cell seen in CSF in an AIDS patients with remarkably little capsule present. These cells may be mistaken for lymphocytes.
India ink preparation of CSF India ink preparation of CSF showing multiple yeasts with large capsules, and narrow buds to smaller daughter cells, typical of C. neoformans
India ink preparation of CSF India ink preparation of CSF showing multiple yeasts with large capsules, and narrow buds to smaller daughter cells, typical of C. neoformans
Histological staining PAS-stain. An example of Aspergillus fumigatus.
Histological staining PAS-stain. An example of Aspergillus fumigatus.

PAS-stainAn example of Aspergillus fumigatus in a patient with chronic granulomatous disease showing a 45 degree branching hypha within a giant cell. Rather bulbous hyphal ends are also seem, which is sometimes found in Aspergillus spp. infections, histologically. x800

Histological staining PAS stain. An example of Aspergillus fumigatus. (PAS-stained) in a patient with chronic granulomatous disease showing a 45 degree branching hypha within a giant cell. Rather bulbous hyphal ends are also seem, which is sometimes found inAspergillus read more...
Histological staining PAS stain. An example of Aspergillus fumigatus. (PAS-stained) in a patient with chronic granulomatous disease showing a 45 degree branching hypha within a giant cell. Rather bulbous hyphal ends are also seem, which is sometimes found inAspergillus read more...

Comparison of GMS and PAS stains. Patient with disseminated Trichosporon spp. infection. Both x60. In the GMS image, substantial background staining of elastin is seen, with more prominent yeasts superimposed. In contrast, the PAS stain shows the tissue morphology, with bright pink yeasts also visible.

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