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Onychomycosis Pt JB attributed to Aspergillus ochraceopetaliformis Image D & E. A case of onychomycosis associated with Aspergillus ochraceopetaliformis as described in Nail infection by Aspergillus ochraceopetaliformis. Med Mycol. 2009 Mar 9:1-5, 2009, Brasch J, Varga J, Jensen JM, Egberts F & Tintelnot K Image A. Culture of Aspergillus ochraceopetaliformis on Sabouraud agar with cycloheximide at 26C , Image B. Culture at higher magnification. , Image C. Aspergillus ochraceopetaliformis conidial heads , Image D. A case of onychomycosis associated with Aspergillus ochraceopetaliformis, Image E. Histology of the infected nail (PAS stain) showing thick fungal elements and septate hyphae within nail material.
Image A. Culture of Aspergillus ochraceopetaliformis on Sabouraud agar with cycloheximide at 26C , Image B. Culture at higher magnification. , Image C. Aspergillus ochraceopetaliformis conidial heads , Image D. A case of onychomycosis associated with Aspergillus ochraceopetaliformis, Image E. Histology of the infected nail (PAS stain) showing thick fungal elements and septate hyphae within nail material. Onychomycosis Pt JB attributed to Aspergillus ochraceopetaliformis Image D & E. A case of onychomycosis associated with Aspergillus ochraceopetaliformis as described in Nail infection by Aspergillus ochraceopetaliformis. Med Mycol. 2009 Mar 9:1-5, 2009, Brasch J, Varga J, Jensen JM, Egberts F & Tintelnot K
Aspergillus versicolor causing onychomycosis Further detailsImage 5. Oral itraconazole pulse therapy was given to the patient (200 mg twice daily for 1 week, with 3 weeks off between successive pulses, for four pulses) and treatment was successful. Image 1. Cultures were grown on malt extract agar. Image kindly provided by Niall Hamilton., Image 2. A Colonies on MEA after one week; B, C conidial heads with tip of conidiophire, x920; D conidial head, x 2330; E conidial heads x920 , Image 3. Pigmentation of Aspergillus versicolor colonies ranged from pale green to greenish-beige, pink-green, dark green and brown. Reverse is usually reddish. The growth rate is usually slow. Cultured on Sabouraud dextrose agar with chloramphenicol. , Image 4. A. versicolor by microscopy showing very long thin conidiophores. , Image 5. A 66 yr old patient in good general health developed onychomycosis. Samples taken from the affected nail were grown by culture and examined by microscopy.
Image 1. Cultures were grown on malt extract agar. Image kindly provided by Niall Hamilton., Image 2. A Colonies on MEA after one week; B, C conidial heads with tip of conidiophire, x920; D conidial head, x 2330; E conidial heads x920 , Image 3. Pigmentation of Aspergillus versicolor colonies ranged from pale green to greenish-beige, pink-green, dark green and brown. Reverse is usually reddish. The growth rate is usually slow. Cultured on Sabouraud dextrose agar with chloramphenicol. , Image 4. A. versicolor by microscopy showing very long thin conidiophores. , Image 5. A 66 yr old patient in good general health developed onychomycosis. Samples taken from the affected nail were grown by culture and examined by microscopy. Aspergillus versicolor causing onychomycosis Further detailsImage 5. Oral itraconazole pulse therapy was given to the patient (200 mg twice daily for 1 week, with 3 weeks off between successive pulses, for four pulses) and treatment was successful.
ALL with disseminated aspergillosis associated with Aspergillus flavus infection This patient was 28 yr old with adult lymphocytic leukaemia. She received induction chemotherapy and this infection developed 2 days after recovering from neutropenia. Image A, Image B, Image C, Image D, Image E, Image F, Image G, Image H, Image I, Image J
Image A, Image B, Image C, Image D, Image E, Image F, Image G, Image H, Image I, Image J ALL with disseminated aspergillosis associated with Aspergillus flavus infection This patient was 28 yr old with adult lymphocytic leukaemia. She received induction chemotherapy and this infection developed 2 days after recovering from neutropenia.

A,B,&C the patient had multiple erythematous lesions on the skin, some of the lesions were papular and showed necrotic tissue.

D,E,F & G MRI brain scans showed multiple bilateral nodular lesions in the frontal, parietal and occipital areas. Lesions were 0.7 - 2cm in diameter involving both peripheral and central areas of the brain. G- illustrates a small well defined lesion in the posterior fossa.

H,I & J CT scans of lungs showing nodular lesions in both lungs with infiltrates. - exhibits the classic halo sign - suggestive of aspergillus infection. J illustrates that some associated pleural effusion was present.

The patient was exposed to high levels of mould during treatment for ALL as a result of a leaking pipe and extensive mould damage to wallcoverings in her home.

Infection of burn wound Close-up image of the lesion on the left thigh showing a mat of hyphae over the wound.
Infection of burn wound Close-up image of the lesion on the left thigh showing a mat of hyphae over the wound.
Patient with allergic fungal sinusitis - staining of eosinophilic mucin Eosinophilic mucin with A. flavus in the nasal cavity. Irregular crust of 2.5 cm from a patient diagnosed as allergic fungal sinusitis. Patient with allergic fungal sinusitis
Patient with allergic fungal sinusitis - staining of eosinophilic mucin Eosinophilic mucin with A. flavus in the nasal cavity. Irregular crust of 2.5 cm from a patient diagnosed as allergic fungal sinusitis. Patient with allergic fungal sinusitis
Patient with allergic fungal sinusitis - staining of eosinophilic mucin GMS stain of eosinophilic mucin reveals a darkly stained dichotomously branched A. flavus hyphae within cellular background. Patient with allergic fungal sinusitis
Patient with allergic fungal sinusitis - staining of eosinophilic mucin GMS stain of eosinophilic mucin reveals a darkly stained dichotomously branched A. flavus hyphae within cellular background. Patient with allergic fungal sinusitis
Light microscopy of mucus sections
Light microscopy of mucus sections

Histological sections of formalin fixed and paraffin embedded mucus, from patients suffering from allergic fungal sinusitis (eosinophilic fungal rhinosinusitis).

Light microscopy of mucus sections
Light microscopy of mucus sections

Histological sections of formalin fixed and paraffin embedded mucus, from patients suffering from allergic fungal sinusitis (eosinophilic fungal rhinosinusitis).

Light microscopy of mucus sections
Light microscopy of mucus sections

Histological sections of formalin fixed and paraffin embedded mucus, from patients suffering from allergic fungal sinusitis (eosinophilic fungal rhinosinusitis).

Allergic fungal sinusitis (or eosinophilic fungal rhinosinusitis) 4 Total obstruction of the sinuses due to inflamed mucosa. (Patient 04)
Allergic fungal sinusitis (or eosinophilic fungal rhinosinusitis) 4 Total obstruction of the sinuses due to inflamed mucosa. (Patient 04)
Allergic fungal sinusitis (or eosinophilic fungal rhinosinusitis) 1 Axial computed tomography (CT) scans of the frontal sinus. A: due to the long lasting pressure of mucus, the bone of the anterior wall of frontal sinus is thinned out and elevated anteriorly, forming a bulge. B: same situation as depicted in fig read more...
Allergic fungal sinusitis (or eosinophilic fungal rhinosinusitis) 1 Axial computed tomography (CT) scans of the frontal sinus. A: due to the long lasting pressure of mucus, the bone of the anterior wall of frontal sinus is thinned out and elevated anteriorly, forming a bulge. B: same situation as depicted in fig read more...
Allergic fungal sinusitis (or eosinophilic fungal rhinosinusitis) 2 Same patient as 1 and 3, frontal CT
Allergic fungal sinusitis (or eosinophilic fungal rhinosinusitis) 3 Frontal CT
Allergic fungal sinusitis (Eosinophilic fungal rhinosinusitis)
Allergic fungal sinusitis (Eosinophilic fungal rhinosinusitis)

Consecutive sections of sinus mucosa from a patient with allergic fungal sinusitis (EFS) top section is stained with H&E and lower one with FITC labelled major basic protein (MBP). The right hand third of the picture shows epithelium and intact eosinophils (with intact granules containing MBP). On the left hand side, clusters of eosinophils have released MBP. Low power MBP is toxic to the sinus epithelium.

Link to protocol for obtaining and fixing specimens.

Allergic fungal sinusitis (Eosinophilic fungal rhinosinusitis)
Allergic fungal sinusitis (Eosinophilic fungal rhinosinusitis)

Consecutive sections of sinus mucosa from a patient with allergic fungal sinusitis (EFS) top section is stained with H&E and lower one with FITC labelled major basic protein (MBP). The right hand third of the picture shows epithelium and intact eosinophils (with intact granules containing MBP). On the left hand side, clusters of eosinophils have released MBP. Low power MBP is toxic to the sinus epithelium.

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