Species

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Aspergillus fumigatus Scanning electron micrographs of A. fumigatus conidia of rodA rodB-26 (d).Size bar, 100 nm.
Aspergillus fumigatus Scanning electron micrographs of A. fumigatus conidia of rodA rodB-26 (d).Size bar, 100 nm. <p>The surface of many fungal conidia is covered by a thin layer of regularly arranged rodlets. This structure appears to favor air buoyancy and dispersion of the conidia by air currents.</p> <p>&nbsp;</p> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">Colonies on&nbsp;CYA&nbsp;40-60 mm&nbsp;diam, plane or lightly wrinkled, low, dense and&nbsp;velutinous&nbsp;or with a sparse,&nbsp;floccose&nbsp;overgrowth; mycelium inconspicuous, white;&nbsp;conidial&nbsp;heads borne in a continuous, densely packed layer,&nbsp;Greyish&nbsp;Turquoise to Dark Turquoise (24-25E-F5); clear&nbsp;exudate&nbsp;sometimes produced in small amounts; reverse pale or greenish. Colonies on MEA 40-60 mm&nbsp;diam, similar to those on&nbsp;CYA&nbsp;but less dense and with&nbsp;conidia&nbsp;in duller&nbsp;colours&nbsp;(24-25E-F3); reverse&nbsp;uncoloured&nbsp;or&nbsp;greyish. Colonies on&nbsp;G25N&nbsp;less than 10 mm&nbsp;diam, sometimes only germination, of white mycelium. No growth at 5°C. At 37°C, colonies covering the available area, i.e. a whole Petri dish in 2 days from a single point&nbsp;inoculum, of similar appearance to those on&nbsp;CYA&nbsp;at 25°C, but with&nbsp;conidial&nbsp;columns longer and&nbsp;conidia&nbsp;darker, greenish grey to pure grey.</p> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">Conidiophores&nbsp;borne from surface&nbsp;hyphae,&nbsp;stipes&nbsp;200-400 µm long, sometimes sinuous, with&nbsp;colourless, thin, smooth walls, enlarging gradually into&nbsp;pyriform&nbsp;vesicles; vesicles 20-30 µm&nbsp;diam, fertile over half or more of the enlarged area, bearing&nbsp;phialides&nbsp;only, the lateral ones characteristically bent so that the tips are approximately parallel to the&nbsp;stipe&nbsp;axis;&nbsp;phialides&nbsp;crowded, 6-8 µm long;&nbsp;conidia&nbsp;spherical to&nbsp;subspheroidal, 2.5-3.0 µm&nbsp;diam, with finely roughened or&nbsp;spinose&nbsp;walls, forming radiate heads at first, then well defined columns of&nbsp;conidia.</p> <h4 style="line-height: normal; font-family: Verdana, Arial, Helvetica, sans-serif; color: rgb(0, 0, 0);"><strong>Distinctive features</strong></h4> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">This distinctive species can be&nbsp;recognised&nbsp;in the unopened Petri dish by its broad,&nbsp;velutinous, bluish colonies bearing characteristic, well defined columns of&nbsp;conidia. Growth at 37°C is exceptionally rapid.&nbsp;Conidial&nbsp;heads are also diagnostic:&nbsp;pyriform&nbsp;vesicles bear crowded&nbsp;phialides&nbsp;which bend to be roughly parallel to the&nbsp;stipe&nbsp;axis. Care should be exercised in handling cultures of this species.</p>
Aspergillus fumigatus
Aspergillus fumigatus

Colonies on CYA 40-60 mm diam, plane or lightly wrinkled, low, dense and velutinous or with a sparse, floccose overgrowth; mycelium inconspicuous, white; conidial heads borne in a continuous, densely packed layer, Greyish Turquoise to Dark Turquoise (24-25E-F5); clear exudate sometimes produced in small amounts; reverse pale or greenish. Colonies on MEA 40-60 mm diam, similar to those on CYA but less dense and with conidia in duller colours (24-25E-F3); reverse uncoloured or greyish. Colonies on G25N less than 10 mm diam, sometimes only germination, of white mycelium. No growth at 5°C. At 37°C, colonies covering the available area, i.e. a whole Petri dish in 2 days from a single point inoculum, of similar appearance to those on CYA at 25°C, but with conidial columns longer and conidia darker, greenish grey to pure grey.

Conidiophores borne from surface hyphae, stipes 200-400 µm long, sometimes sinuous, with colourless, thin, smooth walls, enlarging gradually into pyriform vesicles; vesicles 20-30 µm diam, fertile over half or more of the enlarged area, bearing phialides only, the lateral ones characteristically bent so that the tips are approximately parallel to the stipe axis; phialides crowded, 6-8 µm long; conidia spherical to subspheroidal, 2.5-3.0 µm diam, with finely roughened or spinose walls, forming radiate heads at first, then well defined columns of conidia.

Distinctive features

This distinctive species can be recognised in the unopened Petri dish by its broad, velutinous, bluish colonies bearing characteristic, well defined columns of conidia. Growth at 37°C is exceptionally rapid. Conidial heads are also diagnostic: pyriform vesicles bear crowded phialides which bend to be roughly parallel to the stipe axis. Care should be exercised in handling cultures of this species.

Aspergillus fumigatus Scanning electron micrographs of A. fumigatus conidia of the wild-type G10 strain (a). Size bar, 100 nm.
Aspergillus fumigatus Scanning electron micrographs of A. fumigatus conidia of the wild-type G10 strain (a). Size bar, 100 nm. <p>Scanning electron micrograph of an A.fumigatus conidium showing the hydrophobic rodlets covering the surface. The surface of many fungal conidia is covered by a thin layer of regularly arranged rodlets. This structure appears to favor air buoyancy and dispersion of the conidia by air currents.See Ref: Paris et al APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2003, 1581–1588.</p> <p>&nbsp;</p> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">Colonies on&nbsp;CYA&nbsp;40-60 mm&nbsp;diam, plane or lightly wrinkled, low, dense and&nbsp;velutinous&nbsp;or with a sparse,&nbsp;floccose&nbsp;overgrowth; mycelium inconspicuous, white;&nbsp;conidial&nbsp;heads borne in a continuous, densely packed layer,&nbsp;Greyish&nbsp;Turquoise to Dark Turquoise (24-25E-F5); clear&nbsp;exudate&nbsp;sometimes produced in small amounts; reverse pale or greenish. Colonies on MEA 40-60 mm&nbsp;diam, similar to those on&nbsp;CYA&nbsp;but less dense and with&nbsp;conidia&nbsp;in duller&nbsp;colours&nbsp;(24-25E-F3); reverse&nbsp;uncoloured&nbsp;or&nbsp;greyish. Colonies on&nbsp;G25N&nbsp;less than 10 mm&nbsp;diam, sometimes only germination, of white mycelium. No growth at 5°C. At 37°C, colonies covering the available area, i.e. a whole Petri dish in 2 days from a single point&nbsp;inoculum, of similar appearance to those on&nbsp;CYA&nbsp;at 25°C, but with&nbsp;conidial&nbsp;columns longer and&nbsp;conidia&nbsp;darker, greenish grey to pure grey.</p> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">Conidiophores&nbsp;borne from surface&nbsp;hyphae,&nbsp;stipes&nbsp;200-400 µm long, sometimes sinuous, with&nbsp;colourless, thin, smooth walls, enlarging gradually into&nbsp;pyriform&nbsp;vesicles; vesicles 20-30 µm&nbsp;diam, fertile over half or more of the enlarged area, bearing&nbsp;phialides&nbsp;only, the lateral ones characteristically bent so that the tips are approximately parallel to the&nbsp;stipe&nbsp;axis;&nbsp;phialides&nbsp;crowded, 6-8 µm long;&nbsp;conidia&nbsp;spherical to&nbsp;subspheroidal, 2.5-3.0 µm&nbsp;diam, with finely roughened or&nbsp;spinose&nbsp;walls, forming radiate heads at first, then well defined columns of&nbsp;conidia.</p> <h4 style="line-height: normal; font-family: Verdana, Arial, Helvetica, sans-serif; color: rgb(0, 0, 0);"><strong>Distinctive features</strong></h4> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">This distinctive species can be&nbsp;recognised&nbsp;in the unopened Petri dish by its broad,&nbsp;velutinous, bluish colonies bearing characteristic, well defined columns of&nbsp;conidia. Growth at 37°C is exceptionally rapid.&nbsp;Conidial&nbsp;heads are also diagnostic:&nbsp;pyriform&nbsp;vesicles bear crowded&nbsp;phialides&nbsp;which bend to be roughly parallel to the&nbsp;stipe&nbsp;axis. Care should be exercised in handling cultures of this species.</p>
Aspergillus fumigatus Scanning electron micrographs of A. fumigatus conidia of transformants rodB-02 (b). Size bar, 100 nm.
Aspergillus fumigatus Scanning electron micrographs of A. fumigatus conidia of transformants rodB-02 (b). Size bar, 100 nm. <p>Scanning electron micrograph of an A.fumigatus conidium showing the hydrophobic rodlets covering the surface. The surface of many fungal conidia is covered by a thin layer of regularly arranged rodlets. This structure appears to favor air buoyancy and dispersion of the conidia by air currents.See Ref: Paris et al APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2003, 1581–1588.</p> <p>&nbsp;</p> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">Colonies on&nbsp;CYA&nbsp;40-60 mm&nbsp;diam, plane or lightly wrinkled, low, dense and&nbsp;velutinous&nbsp;or with a sparse,&nbsp;floccose&nbsp;overgrowth; mycelium inconspicuous, white;&nbsp;conidial&nbsp;heads borne in a continuous, densely packed layer,&nbsp;Greyish&nbsp;Turquoise to Dark Turquoise (24-25E-F5); clear&nbsp;exudate&nbsp;sometimes produced in small amounts; reverse pale or greenish. Colonies on MEA 40-60 mm&nbsp;diam, similar to those on&nbsp;CYA&nbsp;but less dense and with&nbsp;conidia&nbsp;in duller&nbsp;colours&nbsp;(24-25E-F3); reverse&nbsp;uncoloured&nbsp;or&nbsp;greyish. Colonies on&nbsp;G25N&nbsp;less than 10 mm&nbsp;diam, sometimes only germination, of white mycelium. No growth at 5°C. At 37°C, colonies covering the available area, i.e. a whole Petri dish in 2 days from a single point&nbsp;inoculum, of similar appearance to those on&nbsp;CYA&nbsp;at 25°C, but with&nbsp;conidial&nbsp;columns longer and&nbsp;conidia&nbsp;darker, greenish grey to pure grey.</p> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">Conidiophores&nbsp;borne from surface&nbsp;hyphae,&nbsp;stipes&nbsp;200-400 µm long, sometimes sinuous, with&nbsp;colourless, thin, smooth walls, enlarging gradually into&nbsp;pyriform&nbsp;vesicles; vesicles 20-30 µm&nbsp;diam, fertile over half or more of the enlarged area, bearing&nbsp;phialides&nbsp;only, the lateral ones characteristically bent so that the tips are approximately parallel to the&nbsp;stipe&nbsp;axis;&nbsp;phialides&nbsp;crowded, 6-8 µm long;&nbsp;conidia&nbsp;spherical to&nbsp;subspheroidal, 2.5-3.0 µm&nbsp;diam, with finely roughened or&nbsp;spinose&nbsp;walls, forming radiate heads at first, then well defined columns of&nbsp;conidia.</p> <h4 style="line-height: normal; font-family: Verdana, Arial, Helvetica, sans-serif; color: rgb(0, 0, 0);"><strong>Distinctive features</strong></h4> <p style="font-family: Verdana, Arial, Helvetica, sans-serif; font-size: 12px; line-height: 1.5; color: rgb(0, 0, 0);">This distinctive species can be&nbsp;recognised&nbsp;in the unopened Petri dish by its broad,&nbsp;velutinous, bluish colonies bearing characteristic, well defined columns of&nbsp;conidia. Growth at 37°C is exceptionally rapid.&nbsp;Conidial&nbsp;heads are also diagnostic:&nbsp;pyriform&nbsp;vesicles bear crowded&nbsp;phialides&nbsp;which bend to be roughly parallel to the&nbsp;stipe&nbsp;axis. Care should be exercised in handling cultures of this species.</p>
A.fumigatus conidium. Scanning electron micrograph of an A.fumigatus conidium showing the fascicles of hydrophobic rodlets covering the surface A.fumigatus conidium. Scanning electron micrograph of an A.fumigatus conidium showing the fascicles of hydrophobic rodlets covering the surface
A.fumigatus conidium. Scanning electron micrograph of an A.fumigatus conidium showing the fascicles of hydrophobic rodlets covering the surface A.fumigatus conidium. Scanning electron micrograph of an A.fumigatus conidium showing the fascicles of hydrophobic rodlets covering the surface
CHEF gel of A. fumigatus chromosomes. CHEF gel of A. fumigatus chromosomes. The electrophoretic karyotype of two isolates of A. fumigatus is shown. The chromosomal bands have been resolved using Bio-Rad CHEF DRII equipment. The sizes of the bands vary between the two isolates and at read more...
CHEF gel of A. fumigatus chromosomes. CHEF gel of A. fumigatus chromosomes. The electrophoretic karyotype of two isolates of A. fumigatus is shown. The chromosomal bands have been resolved using Bio-Rad CHEF DRII equipment. The sizes of the bands vary between the two isolates and at read more... These karyotypes contrast with the published one of AF10 (ATCC 90240) where only three bands in the size range of the S. pombe chromosomes were resolved. However this paper did show that the bottom band at around 1.5 Mb could be resolved into two separate bands using higher field strengths and shorter switching times. In conclusion these data indicate that the electrophoretic karyotype of A. fumigatus consists of at least six chromosomes.
PT JG sternal wound and osteomyelitis following aortic valve replacement These 3 images show non-union of the sternum post-aortic valve replacement as a result of local Aspergillus fumigatus infection. Underlying the sternum is some soft tissue which is presumptively also infection and in the most inferior image, the read more... , ,
, , PT JG sternal wound and osteomyelitis following aortic valve replacement These 3 images show non-union of the sternum post-aortic valve replacement as a result of local Aspergillus fumigatus infection. Underlying the sternum is some soft tissue which is presumptively also infection and in the most inferior image, the read more...
RAPD profiles of 14 A fumigatus isolates from Swedish and New Zealand saw mills, generated with primer R108. Mwt markers (lambda with Pst1) indicated by M. RAPD profiles of 14 A fumigatus isolates from Swedish and New Zealand saw mills, generated with primer R108. Mwt markers (lambda with Pst1) indicated by M.
RAPD profiles of 14 A fumigatus isolates from Swedish and New Zealand saw mills, generated with primer R108. Mwt markers (lambda with Pst1) indicated by M. RAPD profiles of 14 A fumigatus isolates from Swedish and New Zealand saw mills, generated with primer R108. Mwt markers (lambda with Pst1) indicated by M. Kindly reproduced from Birch et al, J Hosp Infect 1995; 30(Suppl):339-351
Aspergillus onychomycosis caused by Aspergillus fumigatus. Aspergillus onychomycosis caused by Aspergillus fumigatus.
Aspergillus onychomycosis caused by Aspergillus fumigatus. Aspergillus onychomycosis caused by Aspergillus fumigatus.
Corneal ulcer due to Aspergillus fumigatus occurring after injury from rose thorn in the garden Corneal ulcer due to Aspergillus fumigatus occurring after injury from rose thorn in the garden
Corneal ulcer due to Aspergillus fumigatus occurring after injury from rose thorn in the garden Corneal ulcer due to Aspergillus fumigatus occurring after injury from rose thorn in the garden
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed Corneal ulcer - gram stain. Corneal scrapings were taken from a 67 yr old farmer presenting with a corneal ulcer of the right eye. A piece of vegetable matter was embedded in the cornea and scrapings were done. Gram stain (500x magnification) showed read more...
Thyroiditis and abscess caused by A. fumigatus. Patient JO. A 14 year old boy with a T cell lymphoma received induction chemotherapy with high dose dexamethasone. At no time during this therapy was he neutropenic. Three weeks into treatment his dexamethasone was reduced and stopped due to gastro-intestinal read more... Image A is an ultrasound image of the thyroid showing an irregular fluid-filled mass in the right lobe of the thyroid gland, with deep haemorrhage. His skin is at the top of the image., Image B is an CT image of his neck demonstrating the abscess in the thyroid., Image C shows his cavitating large lesion in his right lower lobe, taken at same examination that showed his thyroid lesion.
Image A is an ultrasound image of the thyroid showing an irregular fluid-filled mass in the right lobe of the thyroid gland, with deep haemorrhage. His skin is at the top of the image., Image B is an CT image of his neck demonstrating the abscess in the thyroid., Image C shows his cavitating large lesion in his right lower lobe, taken at same examination that showed his thyroid lesion. Thyroiditis and abscess caused by A. fumigatus. Patient JO. A 14 year old boy with a T cell lymphoma received induction chemotherapy with high dose dexamethasone. At no time during this therapy was he neutropenic. Three weeks into treatment his dexamethasone was reduced and stopped due to gastro-intestinal read more...

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