X-ray diffraction evidence for antifungal action of Cassia fistula linn. fruit pulp extract

K. Sharma, G. Dak, L. Nambakkat, V. Kanipotth

Author address: 

Udaipur, IN


Objectives: Cassiafistula Linn is implicated in Indian ethno medicinal literature as having antifungal properties. Hence a study was undertaken to evaluate the inhibitory effect of fruit pulp of this plant against Aspergillusfumigatus. Crude alcohol extract and petroleum ether, benzene, alcohol, chloroform and water fractions of Cassia fistula Linn. fruit pulp were assayed for antifungal activity. X-Ray diffraction studies of all fractions and the treated fungal biomass were done to study the inhibitory effect of the extract on the fungus. Method: Cassiafistula fruit pulp was scraped out and dried at 45°C. Crude alcohol, extract was prepared according to the modified method of Shadomy and Ingroff (1974). Partially purified fractions in petroleum ether, benzene, chloroform, alcohol and water were prepared by successive extraction by reflux method of extraction (Harborne 1984). Antifungal activity was assayed by agar dilution method (Joan Stokes 1975) against Aspergillusfumigatus.15 ml of sterile medium and 1 ml of respective extracts were dispensed in borosilicate glass tubes and autoclaved. Tubes were then inoculated with pure cultures of the test fungus and incubated at 37°C. growth of fungus was observed on 3rd, 6th and 9th day. X-ray diffraction studies were done by exposing the extract/ treated as well as untreated fungal biomass to X-rays from a copper source at an angle of 2 theta for 20 minutes. Result: Crude as well as partially purified fractions of Cassia fistula Linn. fruit pulp inhibited the growth of fungus as inhibition of fungal growth was observed from 3rd day onwards. The magnitude of inhibition differed and maximum inhibition was observed with chloroform fraction. Crystalline nature of extracts was established by presence of sharp peaks in the X-ray diffraction spectra of the various fractions of the extract. Peak identification suggests that these peaks correspond to several primary and secondary metabolites. Several peaks present in the control spectra were absent in the treated spectra suggesting a change in crystalline nature of fungal biomass after treatment. Conclusion: Cassia fistula Linn. fruit pulp possesses antifungal property. Secondary metabolites present in the extract are possibly responsible for the inhibition of fungus. Absence of well-defined peaks in the spectra of treated biomass suggests degradation of functional as well as structural metabolites.

abstract No: 


Full conference title: 

16th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 16th (2006)