Voriconazole Protects A549 Pneumocytes from Invasion by Aspergillus fumigatus

M. NUNEZ, T. HALSEY, A. RYE, and R. WASHBURN.

Abstract: 

Aspergillus fumigatus conidia bind efficiently to monolayers of cultured human A549 cells in vitro, followed by hyphal penetration through the cells (type II pneumocyte cell line; J.inf.dis. 1997;175:146-50). The purpose of the current study was to examine whether the azole antifungal compound, voriconazole, was capable of protecting A549 monolayers against fungal invasion. The experimental protocol was as follows: 1) incubate A549 cell monolayers in the presence or absence of voriconazole (8 mg/ml, 37'C, 16 hr) followed by extensive washing to remove extracellular voriconazole, 2) add resting conidia of A. fumigatus (conidium:A549 ratio = 25:1, 40 min), 3) remove unattached conidia by washing, and 4) Score percentage of A549 cells possessing at least one attached conidium (= attachment index). As expected, conidial attachment in the absence of voriconazole was efficient: the attachment index was 70 ± 7% (n = 4). In contrast, voriconazole significantly protected the cells, decreasing attachment to 21 +/- 3% (n = 4, p
1998

abstract No: 

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Full conference title: 

36th Annual Meeting of the Infectious Diseases Society of America
    • IDSA 36th