In vivo treatment of mice with interferongamma and granulocyte-monocyte colony-stimulating factor (GM-CSF) enhances neutrophil-induced damage of Scedosporium prolificans and Apiospermum hyphae

Gil-Lamaignere, C. , R.V. Vitale, R. Vitale, J. Curfs, P. Verweij, E. Roilides and the Eurofung Network (Thessaloniki, GR; Nijmegen, NL)


Objective: To study the effects of in-vivo treatment with IFN-g, GM-CSF or combination of both cytokines on neutrophil (PMN)-induced damage of Scedosporium prolificans (SP) and Scedosporium apiospermum (SA) hyphae. Scedosporium spp. are hyphomycetes highly resistant to antifungal agents and emerging opportunistic moulds causing serious infections in immunocompromised patients. Methods: BALB/c mice were treated with murine IFN-g, murine GM-CSF or combination of both cytokines daily for 4 days. PMN were elicited to the peritoneal cavity by peptone injection and collected by peritoneal lavage with PBS after 3 hours. For hyphal damage assay, hyphae of either SP or SA were incubated with PMN at effector:target ratio 5:1 in RPMI supplemented with 10% murine pooled serum. Damage of hyphae was assessed using XTT colorimetric assay. Results: Treatment of mice with cytokines significantly enhanced PMN-induced SP-hyphal damage from a mean ± SE 3.0±6.8% for control to 25.6±4.6%, 24.1±3.0% and 40.2±2.2% for IFN-g, GM-CSF and the combination of both cytokines (p

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11th European Congress of Clinical Microbiology and Infectious Diseases
    • ECCMID 11th (2001)