Systemic cryptococcal infections have been successfully treated in the People’s Republic of China using a commercial garlic extract known as allitridium 1 . The intravenous use of this extract has been reported as safe, even after long-term treatment 2 , but few investigations on this method of treatment have been performed in the west. The in-vitro susceptibility of 22 clinical isolates of Cryptococcus neoformans (made up of 15 isolates of C. neoformans var neoformans and 7 isolates of C. neoformans var gattii) were tested against this extract, using the N.C. C.L.S. method (with minor modifications) for broth microdilution. MIC50 and MIC90 results against both cryptococcal varieties was 0.25 µg/mL. The MFC50 result against C. neoformans var neoformans was 16 µg/mL, and against C. neoformans var gattii the result was 8.0 µg/mL. The MFC90 result against both C. neoformans var neoformans and C. neoformans var gattii was 16µg/mL. All tests were repeated, with identical MICs and MFCs within one dilution. The results indicate a consistency of activity by the extract to both varieties of C. neoformans. Our investigations suggest further investigations of the antifungal properties of this extract could be valuable. 1 Shen J, Davis LE, et al. Enhanced Diallyl trisulfide has in vitro synergy with amphotericin B against Cryptococcus neoformans. Medical planta 1996; 62: 415-418. 2 Davis LE, Shen J, Cai Y. Antifungal activity in human cerebrospinal fluid and plasma after intravenous administration of Allium sativum. Antimicrobial agents and chemotherapy 1990; 4: 651-653.
Full conference title:
The 15 th Congress of the International Society for Human and Animal Mycology
- ISHAM 15th (2003)