In vitro evaluation of fibrolytic enzymes as additives for maize (Zea mays L.) silage I. Effects of ensiling temperature, enzyme source and addition level

Dario Colombattoa, Fergus L Moulda, Mahalingeshwara K Bhatb, Richard H Phippsc, Emyr Owena

Author address: 

a Department of Agriculture, The University of Reading, Earley Gate, Reading RG6 6AR, UK b FMS Division, Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK c Centre for Dairy Research, Department of Agriculture, The University of Read


Abstract A series of experiments was completed to investigate the impact of addition of enzymes at ensiling on in vitro rumen degradation of maize silage. Two commercial products, Depol 40 (D, Biocatalysts Ltd., Pontypridd, UK) and Liquicell 2500 (L, Specialty Enzymes and Biochemicals, Fresno, CA, USA), were used. In experiment 1, the pH optima over a pH range 4.06.8 and the stability of D and L under changing pH (4.0, 5.6, 6.8) and temperature (15 and 39°C) conditions were determined. In experiment 2, D and L were applied at three levels to whole crop maize at ensiling, using triplicate 0.5kg capacity laboratory minisilos. A completely randomized design with a factorial arrangement of treatments was used. One set of treatments was stored at room temperature, whereas another set was stored at 40°C during the first 3 weeks of fermentation, and then stored at room temperature. Silages were opened after 120 days. Results from experiment 1 indicated that the xylanase activity of both products showed an optimal pH of about 5.6, but the response differed according to the enzyme, whereas the endoglucanase activity was inversely related to pH. Both products retained at least 70% of their xylanase activity after 48h incubation at 15 or 39°C. In experiment 2, enzymes reduced (P0.05) the final extent of OMD, indicating that this preparation acted on the rumen degradable material. In contrast, silages treated with D had reduced (P

abstract No: 

Page 111-128

Full conference title: 

2003 American Academy of Allergy, Asthma, and Immunology Annual Meeting
    • AAAAI 2003 (59th)